Up-Regulation of S100A8 and S100A9 in Pulmonary Immune Response Induced by a Mycoplasma capricolum subsp. capricolum HN-B Strain.

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  • Additional Information
    • Source:
      Publisher: Molecular Diversity Preservation International Country of Publication: Switzerland NLM ID: 101635614 Publication Model: Electronic Cited Medium: Print ISSN: 2076-2615 (Print) Linking ISSN: 20762615 NLM ISO Abbreviation: Animals (Basel) Subsets: PubMed not MEDLINE
    • Publication Information:
      Original Publication: Basel, Switzerland : Molecular Diversity Preservation International, 2011-
    • Abstract:
      Mycoplasma capricolum subsp. capricolum (Mcc), a member of the Mycoplasma mycoides cluster, has a negative impact on the goat-breeding industry. However, little is known about the pathogenic mechanism of Mcc. This study infected mice using a previously isolated strain, Mcc HN-B. Hematoxylin and eosin staining, RNA sequencing, bioinformatic analyses, RT-qPCR, and immunohistochemistry were performed on mouse lung tissues. The results showed that 235 differentially expressed genes (DEGs) were identified. GO and KEGG enrichment analyses suggested that the DEGs were mainly associated with immune response, defensive response to bacteria, NF-kappa B signaling pathway, natural killer cell-mediated cytotoxicity, and T cell receptor signaling pathway. RT-qPCR verified the expression of Ccl5 , Cd4 , Cd28 , Il2rb , Lck , Lat , Ptgs2 , S100a8 , S100a9 , and Il-33 . The up-regulation of S100A8 and S100A9 at the protein level was confirmed by immunohistochemistry. Moreover, RT-qPCR assays on Mcc HN-B-infected RAW264.7 cells also showed that the expression of S100a8 and S100a9 was elevated. S100A8 and S100A9 not only have diagnostic value in Mcc infection but also hold great significance in clarifying the pathogenic mechanism of Mcc. This study preliminarily elucidates the mechanism of Mcc HN-B-induced lung injury and provides a theoretical basis for further research on Mcc-host interactions.
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    • Grant Information:
      YSPTZX202153 Academician Innovation Platform Project of Hainan Province; CARS-38 Hainan Innovation Center for Academician of Jin Ningyi; Hainan Innovation Center for Academician of Zhang Yong; China Agriculture Research System of MOF and MARA
    • Contributed Indexing:
      Keywords: Mycoplasma capricolum subsp. capricolum; RNA sequencing; S100A8; S100A9; immunohistochemistry
    • Publication Date:
      Date Created: 20240727 Latest Revision: 20240729
    • Publication Date:
      20240729
    • Accession Number:
      PMC11274312
    • Accession Number:
      10.3390/ani14142064
    • Accession Number:
      39061526