Electroacupuncture alleviates myocardial ischemia-reperfusion injury by inhibiting hypothalamic paraventricular nucleus neurons projecting to the rostral ventrolateral medulla.

Publisher: Wiley-Blackwell Country of Publication: France NLM ID: 8918110 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1460-9568 (Electronic) Linking ISSN: 0953816X NLM ISO Abbreviation: Eur J Neurosci Subsets: MEDLINE

Publication: : Oxford : Wiley-Blackwell
Original Publication: Oxford, UK : Published on behalf of the European Neuroscience Association by Oxford University Press, c1989-

Electroacupuncture*/methods ; Paraventricular Hypothalamic Nucleus*/metabolism ; Medulla Oblongata*/metabolism ; Medulla Oblongata*/physiology ; Neurons*/physiology ; Neurons*/metabolism ; Myocardial Reperfusion Injury*/therapy ; Myocardial Reperfusion Injury*/metabolism ; Rats, Sprague-Dawley*; Animals ; Male ; Rats ; Neural Pathways/physiology ; Neural Pathways/metabolism ; Troponin I/metabolism ; Proto-Oncogene Proteins c-fos/metabolism

Accumulating evidence suggests that electroacupuncture (EA) has obvious therapeutic effects and unique advantages in alleviating myocardial ischemia-reperfusion injury (MIRI), while the underlying neuromolecular mechanisms of EA intervention for MIRI have not been fully elucidated. The aim of the study is to investigate the role of the neural pathway of hypothalamic paraventricular nucleus (PVN) neurons projecting to the rostral ventrolateral medulla (RVLM) in the alleviation of MIRI rats by EA preconditioning. MIRI models were established by ligating the left anterior descending coronary artery for 30 min followed by reperfusion for 2 h. Electrocardiogram recording, chemogenetics, enzyme-linked immunosorbent assay, multichannel physiology recording and haematoxylin-eosin and immunofluorescence staining methods were conducted to demonstrate that the firing frequencies of neurons in the PVN and the expression of c-Fos decreased by EA pretreatment. Meanwhile, EA preconditioning significantly reduced the levels of creatine kinase isoenzymes (CK-MB), cardiac troponin I (cTnI) and lactic dehydrogenase (LDH). Virus tracing showed a projection connection between PVN and RVLM. The inhibition of the PVN-RVLM neural pathway could replicate the protective effect of EA pretreatment on MIRI rats. However, the activation of the pathway weakened the effect of EA preconditioning. EA pretreatment alleviated MIRI by regulating PVN neurons projecting to RVLM. This work provides novel evidence of EA pretreatment for alleviating MIRI.
(© 2024 Federation of European Neuroscience Societies and John Wiley & Sons Ltd.)

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2023CXMMTCM019 Research Funds of Center for Xin'an Medicine and Modernization of Traditional Chinese Medicine of IHM; 82074536 Data Center of Management Science, National Natural Science Foundation of China - Peking University; 82104999 Data Center of Management Science, National Natural Science Foundation of China - Peking University; 2022AH020043 Anhui Province University Scientific Research Project; 2022AH030062 Anhui Province University Scientific Research Project; gxgwfx2019025 Anhui Province University Outstanding Top Talent Cultivation Funding Project; gxgnfx2022014 Anhui Province Excellent Youth Backbone Talent Domestic Visit and Training Program; 2108085QH364 Natural Science Foundation of Anhui Province; 2108085Y30 Natural Science Foundation of Anhui Province

Keywords: electroacupuncture (EA); hypothalamic paraventricular nucleus; myocardial ischemia–reperfusion injury (MIRI); neural pathway; rostral ventrolateral medulla

0 (Troponin I)
0 (Proto-Oncogene Proteins c-fos)

Date Created: 20240726 Date Completed: 20240903 Latest Revision: 20240903

20240903

10.1111/ejn.16480

39054660