Optogenetic techniques for understanding the gut peristalsis during chicken embryonic development.

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  • Author(s): Inaba M;Inaba M
  • Source:
    Biochemical Society transactions [Biochem Soc Trans] 2024 Aug 28; Vol. 52 (4), pp. 1727-1735.
  • Publication Type:
    Journal Article; Review; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Portland Press On The Behalf Of The Biochemical Society Country of Publication: England NLM ID: 7506897 Publication Model: Print Cited Medium: Internet ISSN: 1470-8752 (Electronic) Linking ISSN: 03005127 NLM ISO Abbreviation: Biochem Soc Trans Subsets: MEDLINE
    • Publication Information:
      Original Publication: London : Portland Press On The Behalf Of The Biochemical Society
    • Subject Terms:
      Peristalsis*/physiology ; Optogenetics*/methods ; Embryonic Development*; Animals ; Chick Embryo ; Gastrointestinal Tract/physiology ; Gastrointestinal Tract/embryology ; Chickens ; Calcium/metabolism
    • Abstract:
      Gut peristaltic movements transport ingested materials along the gut axis, which is critical for food digestion and nutrient absorption. While a large amount of studies have been devoted to analyzing the physiological functions of peristalsis in adults, little is known about how the peristaltic system is established during embryogenesis. In recent years, the chicken developing gut has emerged as an excellent model, in which specific sites along the gut axis can be genetically labeled enabling live imaging and optogenetic analyses. This review provides an overview of recent progress in optogenetic studies of gut peristalsis. Analyses with an improved channelrhodopsin-2 variant demonstrated that the peristalsis can artificially be generated in the developing gut. These studies unveiled novel functional coordination between different regions along the gut axis. In addition, imaging with GCaMP6s, a genetically encoded calcium indicator, enabled a fine mapping of developmental changes in the peristaltic patterns as Ca2+ signals. These advanced techniques will broaden our knowledge of how embryonic peristalsis is established at the cellular and molecular level, leading to the understanding of physiological and pathological processes in adult peristalsis.
      (© 2024 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.)
    • Contributed Indexing:
      Keywords: Ca2+ imaging; chicken embryo; gut contraction; optogenetics; peristaltic wave
    • Accession Number:
      SY7Q814VUP (Calcium)
    • Publication Date:
      Date Created: 20240725 Date Completed: 20240828 Latest Revision: 20241011
    • Publication Date:
      20250114
    • Accession Number:
      10.1042/BST20231337
    • Accession Number:
      39051133