Gelatin Methacryloyl (GelMA) - 45S5 Bioactive Glass (BG) Composites for Bone Tissue Engineering: 3D Extrusion Printability and Cytocompatibility Assessment Using Human Osteoblasts.

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  • Additional Information
    • Source:
      Publisher: American Chemical Society Country of Publication: United States NLM ID: 101654670 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2373-9878 (Electronic) Linking ISSN: 23739878 NLM ISO Abbreviation: ACS Biomater Sci Eng Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington, DC : American Chemical Society, [2015]-
    • Subject Terms:
      Gelatin*/chemistry ; Tissue Engineering*/methods ; Glass*/chemistry ; Osteoblasts*/drug effects ; Osteoblasts*/cytology ; Ceramics*/chemistry ; Ceramics*/pharmacology ; Methacrylates*/chemistry ; Printing, Three-Dimensional* ; Tissue Scaffolds*/chemistry ; Biocompatible Materials*/chemistry ; Biocompatible Materials*/pharmacology; Humans ; Bone and Bones/drug effects ; Materials Testing ; Cell Proliferation/drug effects
    • Abstract:
      3D extrusion printing has been widely investigated for low-volume production of complex-shaped scaffolds for tissue regeneration. Gelatin methacryloyl (GelMA) is used as a baseline material for the synthesis of biomaterial inks, often with organic/inorganic fillers, to obtain a balance between good printability and biophysical properties. The present study demonstrates how 45S5 bioactive glass (BG) addition and GelMA concentrations can be tailored to develop GelMA composite scaffolds with good printability and buildability. The experimental results suggest that 45S5 BG addition consistently decreases the compression stiffness, irrespective of GelMA concentration, albeit within 20% of the baseline scaffold (without 45S5 BG). The optimal addition of 2 wt % 45S5 BG in 7.5 wt % GelMA was demonstrated to provide the best combination of printability and buildability in the 3D extrusion printing route. The degradation decreases and the swelling kinetics increases with 45S5 BG addition, irrespective of GelMA concentration. Importantly, the dissolution in simulated body fluid over 3 weeks clearly promoted the nucleation and growth of crystalline calcium phosphate particles, indicating the potential of GelMA-45S5 BG to promote biomineralization. The cytocompatibility assessment using human osteoblasts could demonstrate uncompromised cell proliferation or osteogenic marker expression over 21 days in culture for 3D printable 7.5 wt % GelMA -2 wt % 45S5 BG scaffolds when compared to 7.5 wt % GelMA. The results thus encourage further investigations of the GelMA/45S5 BG composite system for bone tissue engineering applications.
    • Contributed Indexing:
      Keywords: 3D extrusion printing; 45S5 bioactive glass; GelMA hydrogel; bone tissue engineering
    • Accession Number:
      9000-70-8 (Gelatin)
      0 (gelatin methacryloyl)
      0 (Methacrylates)
      0 (bioactive glass 45S5)
      0 (Biocompatible Materials)
    • Publication Date:
      Date Created: 20240722 Date Completed: 20240812 Latest Revision: 20240812
    • Publication Date:
      20240813
    • Accession Number:
      10.1021/acsbiomaterials.4c00583
    • Accession Number:
      39038164