Development of a biodegradable prosthesis through tissue engineering, for the organ-replacement or substitution of the extrahepatic bile duct.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Fundación Clínica Médica Sur Country of Publication: Mexico NLM ID: 101155885 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1665-2681 (Print) Linking ISSN: 16652681 NLM ISO Abbreviation: Ann Hepatol Subsets: MEDLINE
    • Publication Information:
      Publication: 2011- : México : Fundación Clínica Médica Sur
      Original Publication: México : Ediciones Medicina y Cultura, 2002-
    • Subject Terms:
      Bile Ducts, Extrahepatic*/surgery ; Tissue Engineering*/methods ; Gelatin* ; Polyesters* ; Absorbable Implants* ; Tissue Scaffolds*; Animals ; Swine ; Materials Testing ; Polylactic Acid-Polyglycolic Acid Copolymer/chemistry ; Cell Proliferation ; Prosthesis Design ; Biocompatible Materials ; Cell Movement ; Cell Adhesion ; Time Factors ; Liver Function Tests ; Nanofibers
    • Abstract:
      Introduction and Objectives: There are different situations in which an extrahepatic bile duct replacement or substitute is needed, such as initial and localized stages of bile duct cancer, agenesis, stenosis, or bile duct disruption.
      Materials and Methods: A prosthesis obtained by electrospinning composed of Poly (D,L-lactide-co-glycolide) (PGLA) - Polycaprolactone (PCL) - Gelatin (Gel) was developed, mechanical and biological tests were carried out to evaluate resistance to tension, biocompatibility, biodegradability, cytotoxicity, morphological analysis and cell culture. The obtained prosthesis was placed in the extrahepatic bile duct of 15 pigs with a 2-year follow-up. Liver function tests and cholangioscopy were evaluated during follow-up.
      Results: Mechanical and biological evaluations indicate that this scaffold is biocompatible and biodegradable. The prosthesis implanted in the experimental model allowed cell adhesion, migration, and proliferation, maintaining bile duct permeability without altering liver function tests. Immunohistochemical analysis indicates the presence of biliary epithelium.
      Conclusions: A tubular scaffold composed of electrospun PGLA-PCL-Gel nanofibers was used for the first time to replace the extrahepatic bile duct in pigs. Mechanical and biological evaluations indicate that this scaffold is biocompatible and biodegradable, making it an excellent candidate for use in bile ducts and potentially in other tissue engineering applications.
      Competing Interests: Conflicts of interest None.
      (Copyright © 2024 Fundación Clínica Médica Sur, A.C. Published by Elsevier España, S.L.U. All rights reserved.)
    • Contributed Indexing:
      Keywords: Bile duct disruption; Biodegradable nanofibers; Electrospinning; Scaffold; Tissue engineering
    • Accession Number:
      9000-70-8 (Gelatin)
      0 (Polyesters)
      24980-41-4 (polycaprolactone)
      1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
      0 (Biocompatible Materials)
    • Publication Date:
      Date Created: 20240721 Date Completed: 20240831 Latest Revision: 20240831
    • Publication Date:
      20240902
    • Accession Number:
      10.1016/j.aohep.2024.101530
    • Accession Number:
      39033929