Using absorbance detection for hs-SV-AUC characterization of adeno-associated virus.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: United States NLM ID: 0370535 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0309 (Electronic) Linking ISSN: 00032697 NLM ISO Abbreviation: Anal Biochem Subsets: MEDLINE
    • Publication Information:
      Publication: <2000- > : San Diego, CA : Elsevier
      Original Publication: Orlando Fl : Academic Press
    • Subject Terms:
      Dependovirus*/genetics ; Dependovirus*/isolation & purification ; Ultracentrifugation*/methods; Humans
    • Abstract:
      Data are presented demonstrating that absorbance detection can be used during high-speed sedimentation velocity analytical ultracentrifugation (hs-SV-AUC) experiments to characterize the size distribution of adeno-associated virus (AAV) drug products accurately. Advantages and limitations of being able to use this detector in this specific type of SV-AUC experiment are discussed.
      Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2024 Elsevier Inc. All rights reserved.)
    • Contributed Indexing:
      Keywords: Adeno-associated virus; Analytical ultracentrifugation; High rotor speeds; High-resolution; Interference detection; Low temperature; Nanoparticles; Sedimentation velocity; Size distribution; Viral vectors
    • Publication Date:
      Date Created: 20240717 Date Completed: 20240820 Latest Revision: 20240820
    • Publication Date:
      20240821
    • Accession Number:
      10.1016/j.ab.2024.115617
    • Accession Number:
      39019206