A flexible glucose biosensor modified by reduced-swelling and conductive zwitterionic hydrogel enzyme membrane.

Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101134327 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-2650 (Electronic) Linking ISSN: 16182642 NLM ISO Abbreviation: Anal Bioanal Chem Subsets: MEDLINE

Original Publication: Heidelberg : Springer-Verlag, 2002-

Biosensing Techniques*/methods ; Glucose Oxidase*/chemistry ; Glucose Oxidase*/metabolism ; Enzymes, Immobilized*/chemistry ; Glucose*/analysis ; Glucose*/chemistry ; Electric Conductivity*; Hydrogels/chemistry ; Humans ; Membranes, Artificial ; Blood Glucose/analysis

This paper reports a flexible glucose biosensor which is modified by a reduced-swelling and conductive zwitterionic hydrogel enzyme membrane that contains two forms of chemical cross-links. One chemical cross-linking is induced by thermal initiators and forms the basal network of the hydrogel. Another cross-linking is achieved by the coordination interactions between the multivalent metal ion Al ³⁺ and anionic group -COO ^- of zwitterionic poly-carboxy betaine (pCBMA), which significantly increase the cross-linking density of the zwitterionic hydrogel, improving the reduced-swelling property and reducing the pore size. The better reduced-swelling property and reduced diameters of pores within the zwitterionic hydrogel make less glucose oxidase (GOx) leakage, thus significantly improving the enzyme membrane's service life. By introducing the Al ³⁺ and Cl ^- , the conductivity of the zwitterionic hydrogel is enhanced approximately 10.4-fold. According to the enhanced conductivity, the reduced-swelling property, and the high GOx loading capacity of the zwitterionic hydrogel, the sensitivity of the biosensor with GOx/pCBMA-Al ³⁺ is significantly improved by 5 times and has a long service life. Finally, the proposed GOx/pCBMA-Al ³⁺ biosensor was applied in non-invasive blood glucose detection on the human body, verifying the capability in practice.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.)

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82072012 National Natural Science Foundation of China; 82102230 National Natural Science Foundation of China; B07014 111 Project of China; 2022YFB3203700 National Key Research and Development Program of China

Keywords: Enzyme membrane; Glucose biosensor; Reduced-swelling hydrogel; Zwitterionic hydrogel

EC 1.1.3.4 (Glucose Oxidase)
0 (Enzymes, Immobilized)
IY9XDZ35W2 (Glucose)
0 (Hydrogels)
0 (Membranes, Artificial)
0 (Blood Glucose)

Date Created: 20240715 Date Completed: 20240817 Latest Revision: 20241015

20241015

10.1007/s00216-024-05429-z

39008068