Thermo-responsive composite nanoparticles based on hydroxybutyl chitosan oligosaccharide: Fabrication, stimulus release and cancer therapy.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE
    • Publication Information:
      Publication: Amsterdam : Elsevier
      Original Publication: Guildford, Eng., IPC Science and Technology Press.
    • Subject Terms:
      Chitosan*/chemistry ; Nanoparticles*/chemistry ; Doxorubicin*/chemistry ; Doxorubicin*/pharmacology ; Oligosaccharides*/chemistry ; Oligosaccharides*/pharmacology ; Drug Liberation* ; Temperature*; Humans ; Drug Carriers/chemistry ; Antineoplastic Agents/pharmacology ; Antineoplastic Agents/chemistry ; HT29 Cells ; Neoplasms/drug therapy ; Hydrogen-Ion Concentration ; Cell Survival/drug effects
    • Abstract:
      Designing thermo-responsive nanocarriers based on biopolymers is fascinating and challenging for cancer therapy. In this study, thermo-responsive composite nanoparticles (CNPs) were prepared using hydroxybutyl chitosan oligosaccharide (HBCOS) and sodium caseinate (SC) via electrostatic interactions and covalent crosslinking. The temperature-responsive behaviors of CNPs were induced by the breakage of hydrogen bonds and the shrinkage of chains in nanoparticles. The CNPs exhibited concentration-independent thermo-responsive behavior, non-adsorption aggregation, and non-hemolysis, suggesting excellent stability and thermo-sensitivity. The initial release rate and final amount of DOX released from CNPs at 42 °C were higher than that at 37 °C, showing a thermo-responsive release, which was also more prominent at lower pH. The release of DOX from CNPs followed first order kinetics based on Fickian diffusion. In vitro cytotoxicity assays confirmed the thermo-responsive antitumor activity of DOX-loaded CNPs as the HT-29 cell viability incubated with DOX-loaded CNPs at 42 °C was significantly lower than that at 37 °C. Cellular uptake experiments proved that DOX-loaded CNPs accumulated in the cytoplasm after being endocytosed and promoted DOX release by increasing environment temperature. This study generated stable thermo-sensitive CNPs based on biopolymers, which can be used as potential nanocarriers for the controlled release of anticancer drugs for cancer therapy.
      Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2024 Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: Composite nanoparticles; Controlled release; Thermo-responsive
    • Accession Number:
      9012-76-4 (Chitosan)
      80168379AG (Doxorubicin)
      0 (Oligosaccharides)
      0 (Drug Carriers)
      0 (Antineoplastic Agents)
    • Publication Date:
      Date Created: 20240714 Date Completed: 20240823 Latest Revision: 20240823
    • Publication Date:
      20240823
    • Accession Number:
      10.1016/j.ijbiomac.2024.133842
    • Accession Number:
      39004251