Lactate promotes fatty acid oxidation by the tricarboxylic acid cycle and mitochondrial respiration in muscles of obese mice.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: American Physiological Society Country of Publication: United States NLM ID: 100901225 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-1563 (Electronic) Linking ISSN: 03636143 NLM ISO Abbreviation: Am J Physiol Cell Physiol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Bethesda, Md. : American Physiological Society,
    • Subject Terms:
      Fatty Acids*/metabolism ; Oxidation-Reduction* ; Muscle, Skeletal*/metabolism ; Citric Acid Cycle*/drug effects ; Lactic Acid*/metabolism ; Obesity*/metabolism ; Mice, Inbred C57BL*; Animals ; Mice ; Male ; Energy Metabolism ; Diet, High-Fat/adverse effects ; Mitochondria, Muscle/metabolism ; Mice, Obese ; Physical Conditioning, Animal ; Cell Respiration ; Mitochondria/metabolism
    • Abstract:
      Lower oxidative capacity in skeletal muscles (SKMs) is a prevailing cause of metabolic diseases. Exercise not only enhances the fatty acid oxidation (FAO) capacity of SKMs but also increases lactate levels. Given that lactate may contribute to tricarboxylic acid cycle (TCA) flux and impact monocarboxylate transporter 1 in the SKMs, we hypothesize that lactate can influence glucose and fatty acid (FA) metabolism. To test this hypothesis, we investigated the mechanism underlying lactate-driven FAO regulation in the SKM of mice with diet-induced obesity (DIO). Lactate was administered to DIO mice immediately after exercise for over 3 wk. We found that increased lactate levels enhanced energy expenditure mediated by fat metabolism during exercise recovery and decreased triglyceride levels in DIO mice SKMs. To determine the lactate-specific effects without exercise, we administered lactate to mice on a high-fat diet (HFD) for 8 wk. Similar to our exercise conditions, lactate increased FAO, TCA cycle activity, and mitochondrial respiration in the SKMs of HFD-fed mice. In addition, under sufficient FA conditions, lactate increased uncoupling protein-3 abundance via the NADH-NAD + shuttle. Conversely, ATP synthase abundance decreased in the SKMs of HFD mice. Taken together, our results suggest that lactate amplifies the adaptive increase in FAO capacity mediated by the TCA cycle and mitochondrial respiration in SKMs under sufficient FA abundance. NEW & NOTEWORTHY Lactate administration post-exercise promotes triglyceride content loss in skeletal muscles (SKMs) and reduced body weight. Lactate enhances fatty acid oxidation in the SKMs of high-fat diet (HFD)-fed mice due to enhanced mitochondrial oxygen consumption. In addition, lactate restores the malate-aspartate shuttle, which is reduced by a HFD, and activates the tricarboxylic acid cycle (TCA) cycle in SKMs. Interestingly, supraphysiological lactate facilitates uncoupling protein-3 expression through NADH/NAD + , which is enhanced under high-fat levels in SKMs.
    • Grant Information:
      2018R1A5A1025511 Engineering Research Centers (ERC); 2022R1A5A2018865 Medical Research Centre (MRC); 2022R1A2C1010188 National Research Foundation (NRF)
    • Contributed Indexing:
      Keywords: TCA cycle; exercise; fatty acid oxidation; lactate; mitochondrial respiration
    • Accession Number:
      0 (Fatty Acids)
      33X04XA5AT (Lactic Acid)
    • Publication Date:
      Date Created: 20240709 Date Completed: 20240823 Latest Revision: 20240823
    • Publication Date:
      20240823
    • Accession Number:
      10.1152/ajpcell.00060.2024
    • Accession Number:
      38981606