Diacylglycerol Signaling in Retinal Ganglion Cells.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
    • Publication Information:
      Publication: Totowa, NJ : Humana Press
      Original Publication: Clifton, N.J. : Humana Press,
    • Subject Terms:
      Retinal Ganglion Cells*/metabolism ; Diglycerides*/metabolism ; Signal Transduction*; Animals ; Mice ; Endocannabinoids/metabolism ; Glycerides/metabolism ; Lipoprotein Lipase/metabolism ; Arachidonic Acids/metabolism ; Mass Spectrometry/methods ; Retina/metabolism
    • Abstract:
      With impaired retinal ganglion cell (RGC) function and eventual RGC death, there is a heightened risk of experiencing glaucoma-induced blindness or other optic neuropathies. Poor RGC efficiency leads to limited transmission of visual signals between the retina and the brain by RGC axons. Increased focus on studying lipid messengers found in neurons such as endocannabinoids (eCBs) has importance due to their potential axonal pathway regenerative properties. 2-Arachidonoylglycerol (2-AG), a common eCB, is synthesized from an sn-1 hydrolysis reaction between diacylglycerol (DAG) and diacylglycerol lipase (DAGL). Examination of DAG production allows for future downstream analysis in relation to DAGL functionality. Here, we describe protocol guidelines for extracting RGCs from mouse retinas and subsequent mass spectrometry analysis of the DAG content present within the RGCs.
      (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
    • References:
      Pasquaré SJ, Chamorro-Aguirre E, Gaveglio VL (2023) The endocannabinoid system in the visual process. J Photochem Photobiol 13:100159. https://doi.org/10.1016/j.jpap.2022.100159. (PMID: 10.1016/j.jpap.2022.100159)
      Stark DT, Caprioli J (2016) Subcellular localization of a 2-arachidonoyl glycerol signaling cassette in retinal ganglion cell axonal growth in vitro. Invest Ophthalmol Vis Sci 57(15):6885–6894. https://doi.org/10.1167/iovs.16-20748. (PMID: 10.1167/iovs.16-2074828002563)
      Reisenberg M et al (2012) The diacylglycerol lipases: structure, regulation and roles in and beyond endocannabinoid signalling. Philos Trans R Soc Lond Ser B Biol Sci 367(1607):3264–3275. https://doi.org/10.1098/rstb.2011.0387. (PMID: 10.1098/rstb.2011.0387)
      Dong W et al (2012) Does diacylglycerol serve as a signaling molecule in plants? Plant Signal Behav 7(4):472–475. https://doi.org/10.4161/psb.19644. (PMID: 10.4161/psb.19644224991713419036)
      Martinez-Torres S et al (2023) Injury-induced activation of the endocannabinoid system promotes axon regeneration. iScience 26(6):106814. https://doi.org/10.1016/j.isci.2023.106814. (PMID: 10.1016/j.isci.2023.1068143723504810205787)
    • Contributed Indexing:
      Keywords: Axon regeneration; Diacylglycerol; Diacylglycerol signaling; Mass spectrometry; Retinal ganglion cells
    • Accession Number:
      0 (Diglycerides)
      0 (Endocannabinoids)
      0 (Glycerides)
      EC 3.1.1.34 (Lipoprotein Lipase)
      0 (Arachidonic Acids)
      8D239QDW64 (glyceryl 2-arachidonate)
    • Publication Date:
      Date Created: 20240708 Date Completed: 20240708 Latest Revision: 20240708
    • Publication Date:
      20240709
    • Accession Number:
      10.1007/978-1-0716-3902-3_18
    • Accession Number:
      38977600