A novel small non-coding RNA 562 mediates the virulence of Pseudomonas plecoglossicida by regulating the expression of fliP, a key component of flagella T3SS.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Academic Press Country of Publication: England NLM ID: 9505220 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9947 (Electronic) Linking ISSN: 10504648 NLM ISO Abbreviation: Fish Shellfish Immunol Subsets: MEDLINE
    • Publication Information:
      Original Publication: London ; New York : Academic Press, c1991-
    • Subject Terms:
      Pseudomonas*/pathogenicity ; Pseudomonas*/genetics ; Pseudomonas*/physiology ; Fish Diseases*/microbiology ; Gene Expression Regulation, Bacterial* ; RNA, Small Untranslated*/genetics ; Flagella*/genetics; Virulence/genetics ; Animals ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; RNA, Bacterial/genetics ; Type III Secretion Systems/genetics ; Bass ; Pseudomonas Infections/immunology
    • Abstract:
      Pseudomonas plecoglossicida is a vital pathogen that poses a substantial risk to aquaculture. Small RNAs (sRNAs) are non-coding regulatory molecules capable of sensing environmental changes and modulating virulence-associated signaling pathways, such as the assembly of flagella. However, the relevant researches on P. plecoglossicida are an urgent need. Here, we report a novel sRNA, sRNA562, which has potential to regulate the post-transcriptional of fliP, a key component of the lateral flagellar type III secretion system. In this study, the effects of sRNA562 on the virulence of P. plecoglossicida and its role in regulating the pathogenic process were investigated through the use of a constructed sRNA562 deletion strain. The deletion of sRNA562 resulted in an up-regulation of fliP in P. plecoglossicida, and leading to increased swarming motility and enhanced the ability of biofilm formation, adhesion and chemotaxis. Subsequent artificial infection experiment demonstrated that the deletion of sRNA562 increased the virulence of P. plecoglossicida towards hybrid grouper, as evidenced by a reduction in survival rate, elevation of tissue bacterial load, and the exacerbation of histopathological damage. Further studies have found that the deletion of sRNA562 lead to an up-regulation of fliP expression during hybrid grouper infection, thereby enhancing bacterial swarming ability and ultimately heightening pathogenicity, leading to a dysregulated host response to infection, tissue damage and eventually death. Our work revealed a sRNA that exerts negative regulation on the expression of lateral flagella in P. plecoglossicida, thereby impacting its virulence. These findings provide a new perspective on the virulence regulation mechanism of P. plecoglossicida, contributing to a more comprehensive understanding in the field of pathogenicity research.
      Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2024 Elsevier Ltd. All rights reserved.)
    • Contributed Indexing:
      Keywords: Flagella; Pseudomonas plecoglossicida; Virulence; sRNA
    • Accession Number:
      0 (RNA, Small Untranslated)
      0 (Bacterial Proteins)
      0 (RNA, Bacterial)
      0 (Type III Secretion Systems)
    • Subject Terms:
      Pseudomonas plecoglossicida
    • Publication Date:
      Date Created: 20240708 Date Completed: 20240720 Latest Revision: 20240820
    • Publication Date:
      20240820
    • Accession Number:
      10.1016/j.fsi.2024.109752
    • Accession Number:
      38977112