Mechanisms of action of berberine hydrochloride in planktonic cells and biofilms of Pseudomonas aeruginosa.

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  • Additional Information
    • Source:
      Publisher: Academic Press Country of Publication: England NLM ID: 8606191 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-1208 (Electronic) Linking ISSN: 08824010 NLM ISO Abbreviation: Microb Pathog Subsets: MEDLINE
    • Publication Information:
      Original Publication: London ; Orlando : Academic Press, c1986-
    • Subject Terms:
      Berberine*/pharmacology ; Pseudomonas aeruginosa*/drug effects ; Pseudomonas aeruginosa*/genetics ; Biofilms*/drug effects ; Biofilms*/growth & development ; Anti-Bacterial Agents*/pharmacology ; Pseudomonas Infections*/microbiology ; Pseudomonas Infections*/drug therapy ; Microbial Sensitivity Tests* ; Reactive Oxygen Species*/metabolism ; Adenosine Triphosphate*/metabolism ; Disease Models, Animal* ; Plankton*/drug effects; Animals ; Mice ; Peritonitis/microbiology ; Peritonitis/drug therapy ; Cell Membrane/drug effects ; Cell Membrane/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism
    • Abstract:
      The increasing prevalence of extensively drug-and pan-drug-resistant Pseudomonas aeruginosa is a major concern for global public health. Therefore, it is crucial to develop novel antimicrobials that specifically target P. aeruginosa and its biofilms. In the present study, we determined that berberine hydrochloride inhibited the growth of planktonic bacteria as well as prevented the formation of biofilms. Moreover, we observed downregulation in the expression of pslA and pelA biofilm-related genes. Compared with existing antibiotics, berberine hydrochloride exhibits multiple modes of action against P. aeruginosa. Our findings suggest that berberine hydrochloride exerts its antimicrobial effects by damaging bacterial cell membranes, generating reactive oxygen species (ROS), and reducing intracellular adenosine triphosphate (ATP) levels. Furthermore, berberine hydrochloride showed minimal cytotoxicity and reduced susceptibility to drug resistance. In a mouse model of peritonitis, it significantly inhibited the growth of P. aeruginosa and exhibited a strong bacteriostatic action. In conclusion, berberine hydrochloride is a safe and effective antibacterial agent that inhibits the growth of P. aeruginosa.
      Competing Interests: Declaration of competing interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
      (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
    • Contributed Indexing:
      Keywords: Berberine hydrochloride; Biofilm; Cell membrane; Pseudomonas aeruginosa; Reactive oxygen species
    • Accession Number:
      0I8Y3P32UF (Berberine)
      0 (Anti-Bacterial Agents)
      0 (Reactive Oxygen Species)
      8L70Q75FXE (Adenosine Triphosphate)
      0 (Bacterial Proteins)
    • Publication Date:
      Date Created: 20240705 Date Completed: 20240722 Latest Revision: 20240722
    • Publication Date:
      20240723
    • Accession Number:
      10.1016/j.micpath.2024.106774
    • Accession Number:
      38969184