ECM derivatized alginate augmenting bio-functionalities of lyophilized mat for skin and liver wound treatment.

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  • Additional Information
    • Source:
      Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 8100316 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-5905 (Electronic) Linking ISSN: 01429612 NLM ISO Abbreviation: Biomaterials Subsets: MEDLINE
    • Publication Information:
      Publication: <1995-> : Amsterdam : Elsevier Science
      Original Publication: [Guilford, England] : IPC Science and Technology Press, 1980-
    • Subject Terms:
      Alginates*/chemistry ; Freeze Drying* ; Extracellular Matrix*/metabolism ; Liver*/metabolism ; Liver*/drug effects ; Wound Healing*/drug effects ; Skin*/drug effects ; Skin*/metabolism ; Tissue Scaffolds*/chemistry; Animals ; Rabbits ; Rats ; Male ; Humans ; Rats, Sprague-Dawley ; Cell Proliferation/drug effects ; Thrombin/metabolism ; Cell Survival/drug effects
    • Abstract:
      Peptides and molecular residues sourced from the fragmentation of the extracellular matrix (ECM) can exacerbate a plethora of cellular functions. We selected a natural ECM-derived complex peptide mixture to functionalize sodium alginate. Three alginate derivatives (sodium alginate conjugated with ECM) SALE-1, SALE-2, and SALE-3 were synthesized using the lowest (10 % w/w), moderate (50 % w/w), and highest (100 % w/w) concentrations of ECM. Thereafter, they were used to fabricate three groups of mat scaffolds EMAT-1 (ECM derivatized alginate thrombin-mat), EMAT-2, and EMAT-3, respectively by the freeze-drying process. To enhance the hemostatic activity, thrombin was loaded onto the scaffolds. Another group, AT, without any derivatized alginate was additionally included in order to comparative analysis. Physical characteristics revealed that the porous mat scaffold showed enhancement in degradation and swelling ability with the increase in ECM content. The higher cell proliferation, migration, and cell viability were noticed in the higher ECM-containing samples EMAT-2 and EMAT-3. In vivo studies using rodent hepatic and rabbit ear models were carried out to ensure the hemostatic ability of the scaffolds. EMAT-2 and EMAT-3 demonstrate excellent liver regeneration ability in rat models. Moreover, the rat cutaneous wound model depicted that EMAT-3 dramatically elevated the skin's healing ability, thereby rendering it an excellent candidate for future clinical application in wound healing.
      Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2024 Elsevier Ltd. All rights reserved.)
    • Contributed Indexing:
      Keywords: Derivatization; ECM; Hemostasis; Proteimics; Wound healing
    • Accession Number:
      0 (Alginates)
      EC 3.4.21.5 (Thrombin)
    • Publication Date:
      Date Created: 20240705 Date Completed: 20240804 Latest Revision: 20240804
    • Publication Date:
      20240805
    • Accession Number:
      10.1016/j.biomaterials.2024.122698
    • Accession Number:
      38968688