Differential root and cell regulation of maize aquaporins by the arbuscular mycorrhizal symbiosis highlights its role in plant water relations.

Publisher: John Wiley & Sons Ltd Country of Publication: United States NLM ID: 9309004 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-3040 (Electronic) Linking ISSN: 01407791 NLM ISO Abbreviation: Plant Cell Environ Subsets: MEDLINE

Publication: Hoboken, NJ : John Wiley & Sons Ltd.
Original Publication: Oxford, UK : Blackwell Scientific Publications

Zea mays*/microbiology ; Zea mays*/genetics ; Zea mays*/physiology ; Zea mays*/metabolism ; Mycorrhizae*/physiology ; Aquaporins*/metabolism ; Aquaporins*/genetics ; Symbiosis* ; Plant Roots*/microbiology ; Plant Roots*/metabolism ; Water*/metabolism ; Gene Expression Regulation, Plant*; Plant Proteins/metabolism ; Plant Proteins/genetics

This study aims to elucidate if the regulation of plant aquaporins by the arbuscular mycorrhizal (AM) symbiosis occurs only in roots or cells colonized by the fungus or at whole root system. Maize plants were cultivated in a split-root system, with half of the root system inoculated with the AM fungus and the other half uninoculated. Plant growth and hydraulic parameters were measured and aquaporin gene expression was determined in each root fraction and in microdissected cells. Under well-watered conditions, the non-colonized root fractions of AM plants grew more than the colonized root fraction. Total osmotic and hydrostatic root hydraulic conductivities (Lo and Lpr) were higher in AM plants than in non-mycorrhizal plants. The expression of most maize aquaporin genes analysed was different in the mycorrhizal root fraction than in the non-mycorrhizal root fraction of AM plants. At the cellular level, differential aquaporin expression in AM-colonized cells and in uncolonized cells was also observed. Results indicate the existence of both, local and systemic regulation of plant aquaporins by the AM symbiosis and suggest that such regulation is related to the availability of water taken up by fungal hyphae in each root fraction and to the plant need of water mobilization.
(© 2024 The Author(s). Plant, Cell & Environment published by John Wiley & Sons Ltd.)

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PHENOLAB 4.0; FEDER 'A way to make Europe'; Ministerio de Ciencia e Innovación

Keywords: arbusculated cell; colonized root fraction; drought; laser microdissection; mycorrhiza; uncolonized root fraction

0 (Aquaporins)
059QF0KO0R (Water)
0 (Plant Proteins)

Date Created: 20240705 Date Completed: 20241001 Latest Revision: 20241001

20241002

10.1111/pce.15029

38965812