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Evaluation of protective immunity induced by a DNA vaccine encoding SAG2 and SRS2 against Toxoplasma gondii infection in mice.
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- Additional Information
- Source:
Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0370374 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-6254 (Electronic) Linking ISSN: 0001706X NLM ISO Abbreviation: Acta Trop Subsets: MEDLINE
- Publication Information:
Publication: Amsterdam : Elsevier
Original Publication: Basel.
- Subject Terms:
Vaccines, DNA*/
immunology ;
Vaccines, DNA*/
genetics ;
Vaccines, DNA*/
administration & dosage ;
Antigens, Protozoan*/
immunology ;
Antigens, Protozoan*/
genetics ;
Protozoan Proteins*/
immunology ;
Protozoan Proteins*/
genetics ;
Toxoplasma*/
immunology ;
Toxoplasma*/
genetics ;
Antibodies, Protozoan*/
blood ;
Protozoan Vaccines*/
immunology ;
Protozoan Vaccines*/
administration & dosage ;
Protozoan Vaccines*/
genetics ;
Immunoglobulin G*/
blood ;
Toxoplasmosis, Animal*/
prevention & control ;
Toxoplasmosis, Animal*/
immunology;
Animals ;
Mice ;
Female ;
Mice, Inbred BALB C ;
Interferon-gamma/
immunology ;
Disease Models, Animal ;
Cell Proliferation ;
Interleukin-4/
immunology ;
Survival Analysis - Abstract:
Toxoplasma gondii is an important protozoan pathogen, which can cause severe diseases in the newborns and immunocompromised individuals. Developing an effective vaccine against Toxoplasma infection is a critically important global health priority. Immunofluorescence staining analysis revealed that TgSAG2 and TgSRS2 are membrane associated and displayed on the surface of the parasite. Immunizations with pBud-SAG2, pBud-SRS2 and pBud-SAG2-SRS2 DNA vaccines significantly increased the production of specific IgG antibodies. Immunization with pBud-SAG2-SRS2 elicited cellular immune response with higher concentrations of IFN-γ and IL-4 compared to the control group. Antigen-specific lymphocyte proliferations in the pBud-SRS2 and pBud-SAG2-SRS2 groups were significantly higher compared to that in the control group. Furthermore, 30 % of mice immunized with pBud-SAG2-SRS2 survived after the challenge infection with virulent T. gondii RH tachyzoites. This study revealed that immunization with pBud-SAG2-SRS2 induced potent immune responses, and has the potential as a promising vaccine candidate for the control of T. gondii infection.
Competing Interests: Declaration of competing interest The authors declare no conflict of interest.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
- Contributed Indexing:
Keywords: DNA vaccine; TgSAG2; TgSRS2; Toxoplasma gondii
- Accession Number:
0 (Vaccines, DNA)
0 (Antigens, Protozoan)
0 (Protozoan Proteins)
0 (Antibodies, Protozoan)
0 (Protozoan Vaccines)
0 (Immunoglobulin G)
133134-61-9 (surface antigen P22, Toxoplasma)
82115-62-6 (Interferon-gamma)
207137-56-2 (Interleukin-4)
- Publication Date:
Date Created: 20240703 Date Completed: 20240729 Latest Revision: 20240906
- Publication Date:
20240906
- Accession Number:
10.1016/j.actatropica.2024.107302
- Accession Number:
38959992
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