Coaxial Electrospun Polycaprolactone/Gelatin Nanofiber Membrane Loaded with Salidroside and Cryptotanshinone Synergistically Promotes Vascularization and Osteogenesis.

Publisher: DOVE Medical Press Country of Publication: New Zealand NLM ID: 101263847 Publication Model: eCollection Cited Medium: Internet ISSN: 1178-2013 (Electronic) Linking ISSN: 11769114 NLM ISO Abbreviation: Int J Nanomedicine Subsets: MEDLINE

Original Publication: Auckland : DOVE Medical Press,

Osteogenesis*/drug effects ; Nanofibers*/chemistry ; Gelatin*/chemistry ; Polyesters*/chemistry ; Glucosides*/chemistry ; Glucosides*/pharmacology ; Phenols*/chemistry ; Phenols*/pharmacology ; Phenanthrenes*/chemistry ; Phenanthrenes*/pharmacology ; Phenanthrenes*/pharmacokinetics ; Phenanthrenes*/administration & dosage ; Neovascularization, Physiologic*/drug effects ; Human Umbilical Vein Endothelial Cells*/drug effects ; Mesenchymal Stem Cells*/drug effects ; Mesenchymal Stem Cells*/cytology ; Rats, Sprague-Dawley*; Animals ; Humans ; Rats ; Male ; Bone Regeneration/drug effects ; Membranes, Artificial ; Coculture Techniques ; Drug Liberation ; Cell Differentiation/drug effects

Background: Salidroside (SAL) is the most effective component of Rhodiola rosea , a traditional Chinese medicine. Cryptotanshinone (CT) is the main fat-soluble extract of Salvia miltiorrhiza , exhibiting considerable potential for application in osteogenesis. Herein, a polycaprolactone/gelatin nanofiber membrane loaded with CT and SAL (PSGC membrane) was successfully fabricated via coaxial electrospinning and characterized.
Methods and Results: This membrane capable of sustained and controlled drug release was employed in this study. Co-culturing the membrane with bone marrow mesenchymal stem cells and human umbilical vein endothelial cells revealed excellent biocompatibility and demonstrated osteogenic and angiogenic capabilities. Furthermore, drug release from the PSGC membrane activated the Wnt/β-catenin signaling pathway and promoted osteogenic differentiation and vascularization. Evaluation of the membrane's vascularization and osteogenic capacities involved transplantation onto a rat's subcutaneous area and assessing rat cranium defects for bone regeneration, respectively. Microcomputed tomography, histological tests, immunohistochemistry, and immunofluorescence staining confirmed the membrane's outstanding angiogenic capacity two weeks post-operation, with a higher incidence of osteogenesis observed in rat cranial defects eight weeks post-surgery.
Conclusion: Overall, the SAL- and CT-loaded coaxial electrospun nanofiber membrane synergistically enhances bone repair and regeneration.
Competing Interests: The authors report no conflicts of interest in this work.
(© 2024 Wu et al.)

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Keywords: coaxial electrospinning; gelatin; vascularization and bone regeneration

M983H6N1S9 (rhodioloside)
9000-70-8 (Gelatin)
0 (Polyesters)
24980-41-4 (polycaprolactone)
0 (Glucosides)
0 (Phenols)
0 (Phenanthrenes)
5E9SXT166N (cryptotanshinone)
0 (Membranes, Artificial)

Date Created: 20240703 Date Completed: 20240703 Latest Revision: 20240704

20240704

PMC11217144

10.2147/IJN.S461141

38957181