The role of miRNA-29b1 on the hypoxia-induced apoptosis in mammalian cardiomyocytes.

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  • Author(s): Dai B;Dai B; Liu H; Liu H; Juan D; Juan D; Wu K; Wu K; Cao R; Cao R
  • Source:
    European journal of histochemistry : EJH [Eur J Histochem] 2024 Jun 27; Vol. 68 (3). Date of Electronic Publication: 2024 Jun 27.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: PagePress Publications Country of Publication: Italy NLM ID: 9207930 Publication Model: Electronic Cited Medium: Internet ISSN: 2038-8306 (Electronic) Linking ISSN: 1121760X NLM ISO Abbreviation: Eur J Histochem Subsets: MEDLINE
    • Publication Information:
      Publication: Jan. 2010- : Pavia : PagePress Publications
      Original Publication: Pavia : Luigi Ponzio, [1992-
    • Subject Terms:
      Apoptosis* ; Cell Hypoxia*/physiology ; MicroRNAs*/metabolism ; MicroRNAs*/genetics ; Myocytes, Cardiac*/metabolism ; Myocytes, Cardiac*/pathology; Animals ; Humans ; Rats ; Cell Line ; Connexin 43/metabolism ; Connexin 43/genetics ; Proto-Oncogene Proteins c-bcl-2/metabolism
    • Abstract:
      Cardiomyocyte apoptosis is a complex biological process involving the interaction of many factors and signaling pathways. In hypoxic environment, cardiomyocytes may trigger apoptosis due to insufficient energy supply, increased production of oxygen free radicals, and disturbance of intracellular calcium ion balance. The present research aimed to investigate the role of microRNA-29b1 (miR-29b1) in hypoxia-treated cardiomyocytes and its potential mechanism involved. We established an in vitro ischemia model using AC16 and H9C2 cardiomyocytes through hypoxia treatment (1% O2, 48 h). Cell apoptosis was evaluated by flow cytometry using Annexin V FITC-PI staining assay. Moreover, we used Western blot and immunofluorescence analysis to determine the expression of Bcl-2, Bax caspase-3 and Cx43 proteins. We found that miR-29b1 protected AC16 and H9C2 cells from hypoxia-induced injury as evidence that miR-29b1 attenuated the effects of hypoxia treatment on AC16 and H9C2 cell apoptosis after hypoxia treatment. In conclusion, our findings suggest that miR-29b1 may have potential cardiovascular protective effects during ischemia-related myocardial injury.
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    • Accession Number:
      0 (Connexin 43)
      0 (MicroRNAs)
      0 (MIRN29 microRNA, rat)
      0 (Proto-Oncogene Proteins c-bcl-2)
      0 (MIRN29B1 microRNA, human)
    • Publication Date:
      Date Created: 20240627 Date Completed: 20240627 Latest Revision: 20240710
    • Publication Date:
      20240710
    • Accession Number:
      PMC11228570
    • Accession Number:
      10.4081/ejh.2024.4021
    • Accession Number:
      38934067