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[Intervention Effect and Mechanism of Regulating MiR-155 on Young Rats with Dysfunction of Blood Coagulation].
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- Additional Information
- Source:
Publisher: Zhongguo shi yan xue za zhi she Country of Publication: China NLM ID: 101084424 Publication Model: Print Cited Medium: Print ISSN: 1009-2137 (Print) Linking ISSN: 10092137 NLM ISO Abbreviation: Zhongguo Shi Yan Xue Ye Xue Za Zhi Subsets: MEDLINE
- Publication Information:
Original Publication: Beijing : Zhongguo shi yan xue za zhi she,
- Subject Terms:
- Abstract:
Objective: To investigate the intervention effect and mechanism of regulating miR-155 on young rats with dysfunction of blood coagulation.
Methods: Twenty-six healthy and clean SD male rats were selected to establish the coagulopathy models. Twenty-four rats successfully established models and were randomly divided into three groups: model group, up-regulated miR-155 group and down-regulated miR-155 group, with 8 rats in each group. The expression of miR-155 was detected by real-time fluorescence quantitative polymerase chain reaction. The changes of coagulation factors and coagulation indicators were observed. Liver pathological tissues were observed by HE staining. The expressions of HMGB1-RAGE/TLRs-NF-κB signaling pathway related proteins were detected by Western blot.
Results: Compared with model group, the expressions of HMGB1, RAGE, TLR2, TLR4 and NF-κB were significantly increased in up-regulated miR-155 group (all P < 0.05), while decreased in down-regulated miR-155 group (all P < 0.05). Compared with model group, the expressions of coagulation factor Ⅱ, Ⅶ, Ⅸ, and Ⅹ were significantly decreased in up-regulated miR-155 group (all P < 0.05), while increased in down-regulated miR-155 group ( P < 0.05). There was no significant difference in the expression of coagulation factor Ⅺ among the three groups ( P >0.05). Compared with model group, the levels of prothrombin time (PT) and activated partial thromboplastin time (APTT) were lower and fibrinogen (FIB) was higher in up-regulated miR-155 group (all P < 0.05), while in the down-regulated miR-155 group they were opposite.
Conclusion: Down-regulation of miR-155 can effectively improve coagulation factors and coagulation indexes and inhibit inflammation in young rats with dysfunction of blood coagulopathy, and the mechanism may be related to HMGB1-RAGE/TLRs-NF-κB signaling pathway.
- Contributed Indexing:
Keywords: microRNA-155; dysfunction of blood coagulation; young rat; mechanism of action; HMGB1-RAGE/TLRs-NF-κB signaling pathway
Local Abstract: [Publisher, Chinese] 调控miR-155对凝血功能障碍模型幼鼠的干预效果及作用机制研究. [Publisher, Chinese] 研究调控miR-155对凝血功能障碍模型幼鼠的干预效果及作用机制。. [Publisher, Chinese] 选取健康、清洁级SD雄性幼鼠26只，建立凝血功能障碍模型，成功24只随机分为模型、上调miR-155和下调miR-155共3组，每组各8只。实时荧光定量聚合酶链式反应检测各组幼鼠miR-155表达，观察各组幼鼠凝血因子水平、凝血指标变化，HE染色观察肝脏病理组织，Western blot法检测HMGB1-RAGE/TLRs-NF-κB信号通路相关蛋白表达。. [Publisher, Chinese] 与模型组比，上调miR-155组HMGB1、RAGE、TLR2、TLR4、NF-κB均明显增高（均 P <0.05），而下调miR-155组表达均明显降低（均 P <0.05）。与模型组比，上调miR-155组凝血因子Ⅱ、Ⅶ、Ⅸ、Ⅹ表达均明显降低（均 P <0.05），而下调miR-155组均表达升高（均 P <0.05）。三组凝血因子Ⅺ表达差异比较无统计学意义（ P >0.05）。与模型组比，上调miR-155组凝血酶原时间、活化部分凝血活酶水平较低，纤维蛋白原水平较高（均 P <0.05），而下调miR-155组正好相反。. [Publisher, Chinese] 下调miR-155能有效改善凝血功能障碍幼鼠凝血因子水平及凝血指标，抑制炎症反应，其作用机制可能与HMGB1-RAGE/TLRs-NF-κB信号通路有关。.
- Accession Number:
0 (MicroRNAs)
0 (NF-kappa B)
0 (HMGB1 Protein)
0 (MIRN155 microRNA, rat)
0 (Toll-Like Receptor 4)
0 (Blood Coagulation Factors)
0 (Toll-Like Receptor 2)
- Publication Date:
Date Created: 20240627 Date Completed: 20240627 Latest Revision: 20240716
- Publication Date:
20240717
- Accession Number:
10.19746/j.cnki.issn.1009-2137.2024.03.031
- Accession Number:
38926979
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