Starvation-induced changes in the proteome and transcriptome of the salivary glands of leech (Hirudo nipponia).

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  • Additional Information
    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science
    • Subject Terms:
      Salivary Glands*/metabolism ; Transcriptome* ; Proteome*/metabolism ; Starvation*/metabolism ; Starvation*/genetics ; Leeches*/genetics ; Leeches*/metabolism; Animals ; Gene Expression Profiling
    • Abstract:
      Hirudo nipponia is an important medicinal animal in China. Its salivary gland secretions contain a variety of protein bioactive substances. Investigations of its salivary glands are of great significance in the study of the medicinal value and mechanism of leech secretions. Illumina RNA-Seq technology was used to perform transcriptome sequencing of salivary gland tissue of H. nipponia under starvation (D30) and fed (D0) states. A total of 2,650 differentially expressed genes (DEGs) were screened. Using the label-free protein quantification technique and bioinformatics analysis, the expression of differentially expressed proteins (DEPs) in the salivary gland tissue of H. nipponia was compared. A total of 2,021 proteins were identified, among which 181 proteins were differentially expressed between the starvation and fed states, with 72 significantly upregulated and 109 significantly downregulated. The salivary glands of H. nipponia synthesized protein-based active substances after 30 days of starvation and adapted to the starvation environment by weakening respiratory activity and reducing metabolic activity to reduce energy expenditure. Energy was produced by glycolysis and the tricarboxylic acid cycle for the synthesis of substances such as antibiotics. This study combined transcriptome and proteome sequencing data to provide a data reference for an in-depth study of the regulatory mechanism of salivary gland secretions of H. nipponia under starvation stress by analyzing DEGs and DEPs.
      Competing Interests: The authors have declared that no competing interests exist.
      (Copyright: © 2024 Cai et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
    • References:
      Carbohydr Polym. 2022 Feb 1;277:118838. (PMID: 34893255)
      Mol Omics. 2018 Oct 8;14(5):352-361. (PMID: 30239540)
      Ticks Tick Borne Dis. 2015 Apr;6(3):424-34. (PMID: 25825233)
      Genes Dis. 2022 Jun 13;10(4):1582-1595. (PMID: 37397563)
      Cell Signal. 2014 Jul;26(7):1539-48. (PMID: 24703938)
      Biol Chem. 2003 Sep;384(9):1333-9. (PMID: 14515997)
      Int J Low Extrem Wounds. 2022 Dec;21(4):425-431. (PMID: 32815407)
      J Exp Med. 1941 Mar 31;73(4):493-506. (PMID: 19871093)
      Comp Biochem Physiol B Biochem Mol Biol. 2004 Apr;137(4):443-54. (PMID: 15081996)
      Zool Res. 2019 May 18;40(3):205-210. (PMID: 31011131)
      Chemotherapy. 2006;52(3):158-60. (PMID: 16636539)
      Haemostasis. 1991;21 Suppl 1:3-10. (PMID: 1894194)
      Bioinformatics. 2006 Jul 1;22(13):1658-9. (PMID: 16731699)
      Bioorg Khim. 2008 May-Jun;34(3):337-43. (PMID: 18672682)
      Biotechnol Lett. 2021 Jan;43(1):271-277. (PMID: 32910358)
      J Neurobiol. 1999 Aug;40(2):244-53. (PMID: 10413454)
      Blood. 1995 Feb 1;85(3):705-11. (PMID: 7833474)
      Blood. 1995 Feb 1;85(3):712-9. (PMID: 7833475)
      J Vasc Surg Cases Innov Tech. 2023 Aug 18;9(4):101296. (PMID: 37767354)
      Ann Parasitol. 2014;60(2):89-92. (PMID: 25115059)
      Nature. 2020 Jul;583(7815):303-309. (PMID: 32612236)
      Indian J Pharm Sci. 2013 Mar;75(2):127-37. (PMID: 24019559)
      Development. 2020 May 28;147(10):. (PMID: 32467294)
      J Biochem. 2001 Sep;130(3):431-8. (PMID: 11530020)
      Nat Cell Biol. 2013 Apr;15(4):417-29. (PMID: 23524952)
      Chin J Nat Med. 2019 Aug;17(8):591-599. (PMID: 31472896)
      Sci Total Environ. 2019 Dec 10;695:133897. (PMID: 31425978)
      Biomedicines. 2020 Apr 27;8(5):. (PMID: 32349294)
      Biomed Khim. 2008 Mar-Apr;54(2):127-39. (PMID: 18522215)
      Biol Chem Hoppe Seyler. 1986 Dec;367(12):1235-42. (PMID: 3828073)
    • Accession Number:
      0 (Proteome)
    • Publication Date:
      Date Created: 20240626 Date Completed: 20240626 Latest Revision: 20240628
    • Publication Date:
      20240628
    • Accession Number:
      PMC11207150
    • Accession Number:
      10.1371/journal.pone.0304453
    • Accession Number:
      38923974