Salt assisted liquid-liquid extraction combined with dispersive liquid-liquid microextraction for the determination of 24 regulated polycyclic aromatic hydrocarbons in human serum.

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  • Additional Information
    • Source:
      Publisher: Elsevier Science Country of Publication: England NLM ID: 8309336 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-264X (Electronic) Linking ISSN: 07317085 NLM ISO Abbreviation: J Pharm Biomed Anal Subsets: MEDLINE
    • Publication Information:
      Publication: <2006->: London : Elsevier Science
      Original Publication: Oxford ; New York : Pergamon Press, c1983-
    • Subject Terms:
      Liquid Phase Microextraction*/methods ; Polycyclic Aromatic Hydrocarbons*/blood ; Polycyclic Aromatic Hydrocarbons*/analysis ; Solvents*/chemistry ; Liquid-Liquid Extraction*/methods; Humans ; Limit of Detection ; Salts/chemistry ; Chromatography, High Pressure Liquid/methods ; Chromatography, Liquid/methods
    • Abstract:
      Polycyclic aromatic hydrocarbons (PAHs) are persistent organic pollutants of great concern due to their carcinogenicity and mutagenicity. Their determination in human serum, particularly in at-risk populations, is necessary but difficult because they are distributed over a wide range of polarity and are present at trace level. A new method combining salting-out assisted liquid-liquid extraction (SALLE) and dispersive liquid-liquid microextraction with solidification of floating organic drop (DLLME-SFO) adapted to a reduced volume of sample (100 µl) was developed to determine 24 PAHs in human serum. Some key parameters of DLLME-SFO (volume of extraction solvent, ratio of extraction/dispersive solvent volumes, and salt addition) were first studied by applying it to spiked pure water. For its application to serum, a sample treatment step involving SALLE was optimized in terms of nature and content of salts and applied upstream of DLLME-SFO. It was applied to the extraction of 24 regulated PAHs from spiked serum followed by an analysis by liquid chromatography coupled with UV and fluorescence detection. The extraction recoveries ranged from 48.2 and 116.0 % (relative standard deviations: 2.0-14.6 %, n=5-9), leading to limits of quantification of PAHs in human serum from 0.04 to 1.03 µg/L using fluorescence detection and from 10 to 40 µg/L using UV detection. This final method combining SALLE and DLLME-SFO showed numerous advantages such as no evaporation step, high efficiency and low solvent-consumption and will be useful for monitoring PAHs in low volumes of serum.
      Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper
      (Copyright © 2024 Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: Dispersive liquid-liquid microextraction; Liquid chromatography; Polycyclic aromatic hydrocarbons; Salting-out liquid-liquid extraction; Serum
    • Accession Number:
      0 (Polycyclic Aromatic Hydrocarbons)
      0 (Solvents)
      0 (Salts)
    • Publication Date:
      Date Created: 20240622 Date Completed: 20240718 Latest Revision: 20240718
    • Publication Date:
      20240719
    • Accession Number:
      10.1016/j.jpba.2024.116319
    • Accession Number:
      38908235