Production of lactulose from lactose using a novel cellobiose 2-epimerase from Clostridium disporicum.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: United States NLM ID: 8003761 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0909 (Electronic) Linking ISSN: 01410229 NLM ISO Abbreviation: Enzyme Microb Technol Subsets: MEDLINE
    • Publication Information:
      Publication: New York, NY : Elsevier
      Original Publication: [Guildford, Eng.] IPC Science and Technology Press.
    • Subject Terms:
      Lactulose*/metabolism ; Lactulose*/biosynthesis ; Lactose*/metabolism ; Clostridium*/enzymology ; Clostridium*/genetics ; Escherichia coli*/genetics ; Escherichia coli*/metabolism; Bacterial Proteins/metabolism ; Bacterial Proteins/genetics ; Carbohydrate Epimerases/genetics ; Carbohydrate Epimerases/metabolism ; Hydrogen-Ion Concentration ; Recombinant Proteins/metabolism ; Recombinant Proteins/genetics ; Cellobiose/metabolism ; Temperature
    • Abstract:
      Lactulose is a semisynthetic nondigestive sugar derived from lactose, with wide applications in the food and pharmaceutical industries. Its biological production routes which use cellobiose 2-epimerase (C2E) as the key enzyme have attracted widespread attention. In this study, a set of C2Es from different sources were overexpressed in Escherichia coli to produce lactulose. We obtained a novel and highly efficient C2E from Clostridium disporicum (CDC2E) to synthesize lactulose from lactose. The effects of different heat treatment conditions, reaction pH, reaction temperature, and substrate concentrations were investigated. Under the optimum biotransformation conditions, the final concentration of lactulose was up to 1.45 M (496.3 g/L), with a lactose conversion rate of 72.5 %. This study provides a novel C2E for the biosynthesis of lactulose from low-cost lactose.
      Competing Interests: Conflict of Interest The authors confirm that there is no conflict of interest regarding this article.
      (Copyright © 2024 Elsevier Inc. All rights reserved.)
    • Contributed Indexing:
      Keywords: Cellobiose 2-epimerase; Clostridium disporicum; Lactulose; Whole-cell biocatalyst
    • Accession Number:
      4618-18-2 (Lactulose)
      J2B2A4N98G (Lactose)
      0 (Bacterial Proteins)
      EC 5.1.3.- (Carbohydrate Epimerases)
      0 (Recombinant Proteins)
      16462-44-5 (Cellobiose)
    • Publication Date:
      Date Created: 20240618 Date Completed: 20240805 Latest Revision: 20240912
    • Publication Date:
      20240913
    • Accession Number:
      10.1016/j.enzmictec.2024.110466
    • Accession Number:
      38889605