Exploring Preservation Modalities in a Split Human Pancreas Model to Investigate the Effect on the Islet Isolation Outcomes.

Publisher: Wolters Kluwer Country of Publication: United States NLM ID: 101651609 Publication Model: eCollection Cited Medium: Print ISSN: 2373-8731 (Print) Linking ISSN: 23738731 NLM ISO Abbreviation: Transplant Direct Subsets: PubMed not MEDLINE

Original Publication: [Philadelphia, Pa.] : Wolters Kluwer, [2015]-

Background: In islet transplantation, the use of dynamic hypothermic preservation techniques is a current challenge. This study compares the efficacy of 3 pancreas preservation methods: static cold storage, hypothermic machine perfusion (HMP), and oxygenated HMP.
Methods: A standardized human pancreas split model was employed using discarded organs from both donation after brain death (n = 15) and donation after circulatory death (DCD) (n = 9) donors. The pancreas head was preserved using static cold storage (control group), whereas the tail was preserved using the 3 different methods (study group). Data on donor characteristics, pancreas histology, isolation outcomes, and functional tests of isolated islets were collected.
Results: Insulin secretory function evaluated by calculating stimulation indices and total amount of secreted insulin during high glucose stimulation (area under the curve) through dynamic perifusion experiments was similar across all paired groups from both DCD and donation after brain death donors. In our hands, islet yield (IEQ/g) from the pancreas tails used as study groups was higher than that of the pancreas heads as expected although this difference did not always reach statistical significance because of great variability probably due to suboptimal quality of organs released for research purposes. Moreover, islets from DCD organs had greater purity than controls ( P ≤ 0.01) in the HMP study group. Furthermore, our investigation revealed no significant differences in pancreas histology, oxidative stress markers, and apoptosis indicators.
Conclusions: For the first time, a comparative analysis was conducted, using a split model, to assess the effects of various preservation methods on islets derived from pancreas donors. Nevertheless, no discernible variances were observed in terms of islet functionality, histological attributes, or isolation efficacy. Further investigations are needed to validate these findings for clinical application.
(Copyright © 2024 The Author(s). Transplantation Direct. Published by Wolters Kluwer Health, Inc.)

Adv Exp Med Biol. 2010;654:683-710. (PMID: 20217520)
PLoS One. 2011;6(11):e27445. (PMID: 22102895)
Transplantation. 2024 Jan 1;108(1):22-44. (PMID: 37026713)
J Hepatol. 2014 Apr;60(4):765-72. (PMID: 24295869)
Am J Transplant. 2015 Jan;15(1):161-9. (PMID: 25521639)
Diabetes. 1988 Apr;37(4):413-20. (PMID: 3288530)
Transplantation. 2003 May 15;75(9):1423-9. (PMID: 12792491)
Lancet. 2020 Nov 21;396(10263):1653-1662. (PMID: 33220737)
PLoS One. 2021 May 3;16(5):e0251055. (PMID: 33939760)
Islets. 2009 Sep-Oct;1(2):129-36. (PMID: 20606719)
Front Endocrinol (Lausanne). 2023 Jun 30;14:1195545. (PMID: 37455917)
Transplant Proc. 2010 Jul-Aug;42(6):2016-9. (PMID: 20692396)
Nature. 2014 Nov 20;515(7527):431-435. (PMID: 25383517)
Nephrol Dial Transplant. 2012 Dec;27(12):4458-63. (PMID: 22844103)
Transplant Rev (Orlando). 2018 Jul;32(3):127-131. (PMID: 29566990)
Transplant Direct. 2018 Sep 07;4(10):e388. (PMID: 30498765)
Artif Organs. 2023 Apr;47(4):777-785. (PMID: 36461753)
Am J Transplant. 2020 Aug;20(8):2030-2043. (PMID: 32012434)
Cell Transplant. 2010;19(5):613-28. (PMID: 20149300)
Cell Transplant. 2017 May 9;26(5):901-911. (PMID: 27938490)
Sci Rep. 2015 Sep 29;5:14634. (PMID: 26417671)
Cell Transplant. 2013;22(8):1477-83. (PMID: 23044229)
Transplantation. 2012 Apr 15;93(7):693-702. (PMID: 22318245)
PLoS One. 2013 Jun 24;8(6):e67454. (PMID: 23826303)
Transpl Int. 2016 Jul;29(7):798-806. (PMID: 26340064)
Arch Biochem Biophys. 1979 Jul;195(2):485-93. (PMID: 224819)
Islets. 2010 May-Jun;2(3):135-45. (PMID: 20657742)
Nat Rev Endocrinol. 2017 May;13(5):268-277. (PMID: 27834384)
Am J Transplant. 2019 Mar;19(3):752-762. (PMID: 30171799)
Pancreas. 2018 May/Jun;47(5):533-543. (PMID: 29621044)
J Histochem Cytochem. 2005 Sep;53(9):1087-97. (PMID: 15923354)
Pancreas. 2010 Jan;39(1):57-63. (PMID: 19745778)
Transplantation. 2022 Jul 1;106(7):1450-1454. (PMID: 35411875)
Transplantation. 2019 Jan;103(1):160-167. (PMID: 30095738)
Transplantation. 2022 Sep 1;106(9):1770-1776. (PMID: 36001489)
Islets. 2012 Mar-Apr;4(2):167-72. (PMID: 22653677)
Curr Opin Organ Transplant. 2016 Aug;21(4):386-92. (PMID: 27096564)
Transplant Proc. 2004 May;36(4):1050-1. (PMID: 15194365)
Exp Clin Transplant. 2010 Mar;8(1):55-60. (PMID: 20199372)
J Surg Res. 1983 May;34(5):493-504. (PMID: 6341715)
Transplantation. 2020 Apr;104(4):731-743. (PMID: 31764761)
Am J Transplant. 2010 Apr;10(4):837-845. (PMID: 20121753)
Arch Surg. 1980 Jan;115(1):95-8. (PMID: 6766062)
Transplant Proc. 2005 Oct;37(8):3501-4. (PMID: 16298642)
Transplantation. 2023 Feb 1;107(2):326-340. (PMID: 35939388)
Transplantation. 2020 Jan;104(1):190-196. (PMID: 31365472)
Cell Med. 2012 Jan 1;2(3):105-110. (PMID: 24083059)
Transplantation. 2021 Aug 1;105(8):1685-1694. (PMID: 33606487)
PLoS One. 2014 Feb 14;9(2):e88521. (PMID: 24551114)
Transpl Int. 2021 Aug;34(8):1397-1407. (PMID: 34036616)
Cancers (Basel). 2019 Oct 10;11(10):. (PMID: 31658694)

Date Created: 20240617 Latest Revision: 20240618

20240618

PMC11177812

10.1097/TXD.0000000000001654

38881744