A multicrosslinked network composite hydrogel scaffold based on DLP photocuring printing for nasal cartilage repair.

Publisher: Wiley Country of Publication: United States NLM ID: 7502021 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-0290 (Electronic) Linking ISSN: 00063592 NLM ISO Abbreviation: Biotechnol Bioeng Subsets: MEDLINE

Publication: <2005->: Hoboken, NJ : Wiley
Original Publication: New York, Wiley.

Printing, Three-Dimensional* ; Hydrogels*/chemistry ; Rats, Sprague-Dawley* ; Tissue Scaffolds*/chemistry ; Nasal Cartilages*; Animals ; Rats ; Chondrocytes/cytology ; Tissue Engineering ; Hyaluronic Acid/chemistry ; Gelatin/chemistry ; Bioprinting/methods

Natural hydrogels are widely employed in tissue engineering and have excellent biodegradability and biocompatibility. Unfortunately, the utilization of such hydrogels in the field of three-dimensional (3D) printing nasal cartilage is constrained by their subpar mechanical characteristics. In this study, we provide a multicrosslinked network hybrid ink made of photocurable gelatin, hyaluronic acid, and acrylamide (AM). The ink may be processed into intricate 3D hydrogel structures with good biocompatibility and high stiffness properties using 3D printing technology based on digital light processing (DLP), including intricate shapes resembling noses. By varying the AM content, the mechanical behavior and biocompatibility of the hydrogels can be adjusted. In comparison to the gelatin methacryloyl (GelMA)/hyaluronic acid methacryloyl (HAMA) hydrogel, adding AM considerably enhances the hydrogel's mechanical properties while also enhancing printing quality. Meanwhile, the biocompatibility of the multicrosslinked network hydrogels and the development of cartilage were assessed using neonatal Sprague-Dawley (SD) rat chondrocytes (CChons). Cells sown on the hydrogels considerably multiplied after 7 days of culture and kept up the expression of particular proteins. Together, our findings point to GelMA/HAMA/polyacrylamide (PAM) hydrogel as a potential material for nasal cartilage restoration. The photocuring multicrosslinked network ink composed of appropriate proportions of GelMA/HAMA/PAM is very suitable for DLP 3D printing and will play an important role in the construction of nasal cartilage, ear cartilage, articular cartilage, and other tissues and organs in the future. Notably, previous studies have not explored the application of 3D-printed GelMA/HAMA/PAM hydrogels for nasal cartilage regeneration.
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Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi; Key Clinical Projects of Peking University Third Hospital; Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering; National Natural Science Foundation of China; Shanxi Provincial Key Medical Scientific Research Project; Shanxi Provincial Basic Research Project

Keywords: GelMA; HAMA; biocompatible; biodegradable; nasal cartilage repair; natural‐synthetic polymer biohybrid hydrogel

0 (Hydrogels)
9004-61-9 (Hyaluronic Acid)
9000-70-8 (Gelatin)

Date Created: 20240615 Date Completed: 20240814 Latest Revision: 20240814

20240814

10.1002/bit.28769

38877732