Borrelia theileri infections in Rhipicephalus annulatus ticks from the north of Iran.

Publisher: Kluwer Academic Publishers Country of Publication: Netherlands NLM ID: 8507436 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1572-9702 (Electronic) Linking ISSN: 01688162 NLM ISO Abbreviation: Exp Appl Acarol Subsets: MEDLINE

Publication: 1999- : Dordrecht : Kluwer Academic Publishers
Original Publication: Amsterdam ; New York : Elsevier, [c1985-

Borrelia*/isolation & purification ; Borrelia*/genetics ; Rhipicephalus*/microbiology; Animals ; Iran ; Female ; Male ; Nymph/microbiology ; Nymph/growth & development ; Phylogeny ; Real-Time Polymerase Chain Reaction ; Cattle

Ticks serve as vectors and reservoirs of various Borrelia species, potentially causing diseases in humans and animals. Mazandaran, a fertile green land in northern Iran, provides ample grazing grounds for livestock and harbors at least 26 hard tick species. This study investigated Borrelia infection in hard ticks from forest areas in this region and compared their genetic identity with the species data in the GenBank database. A total of 2,049 ticks were collected manually from mammalian hosts or using dragging and flagging methods. These ticks were then grouped into 190 pools and 41 individuals based on host, species, developmental stage, and gender. A real-time PCR (qPCR) detected Borrelia DNA in 26 pools from female, male, and nymph of Rhipicephalus annulatus (n = 17) and Ixodes ricinus (n = 9) ticks and one individual female Haemaphysalis punctata tick. The generated partial flaB and glpQ sequences from qPCR-positive Rh. annulatus ticks exhibited the highest identities of 98.1-100% and 98.2% with Borrelia theileri and closely related undefined isolates. Additionally, in phylogenetic analysis, these sequences clustered within well-supported clades with B. theileri and the closely related undefined isolates from various geographic regions, confirming the presence of B. theileri in the north of Iran. Divergence in B. theileri flaB and glpQ sequences across various geographical areas suggests potential subspeciation driven by adaptations to different tick species. This divergence in our flaB sequences implies the possible introduction of B. theileri-infected ticks from different geographical origins into Iran.
(© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

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Grant No.: 1250 Pasteur Institute of Iran

Keywords: Borrelia theileri; Rhipicephalus annulatus ticks; Iran; Mazandaran; Molecular detection; Phylogenetic analysis

Date Created: 20240613 Date Completed: 20240617 Latest Revision: 20240626

20240626

10.1007/s10493-024-00924-5

38869723