I-FABP protein/mRNA and IL-6 as biomarkers of intestinal barrier dysfunction in neonates with necrotizing enterocolitis and SPF BALB/c mouse models.

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  • Additional Information
    • Source:
      Publisher: Sage Publications Country of Publication: England NLM ID: 0346411 Publication Model: Print Cited Medium: Internet ISSN: 1473-2300 (Electronic) Linking ISSN: 03000605 NLM ISO Abbreviation: J Int Med Res Subsets: MEDLINE
    • Publication Information:
      Publication: Nov. 2012- : London : Sage Publications
      Original Publication: Northampton, Eng., Cambridge Medical Publications ltd.
    • Subject Terms:
      Enterocolitis, Necrotizing*/metabolism ; Enterocolitis, Necrotizing*/blood ; Enterocolitis, Necrotizing*/pathology ; Enterocolitis, Necrotizing*/genetics ; Enterocolitis, Necrotizing*/diagnosis ; Fatty Acid-Binding Proteins*/blood ; Fatty Acid-Binding Proteins*/genetics ; Fatty Acid-Binding Proteins*/metabolism ; Interleukin-6*/blood ; Interleukin-6*/genetics ; Biomarkers*/blood ; Biomarkers*/metabolism ; RNA, Messenger*/genetics ; RNA, Messenger*/metabolism ; RNA, Messenger*/blood ; Disease Models, Animal* ; Mice, Inbred BALB C*; Animals ; Infant, Newborn ; Humans ; Mice ; Male ; Female ; Animals, Newborn ; Intestinal Mucosa/metabolism ; Intestinal Mucosa/pathology ; Ileum/metabolism ; Ileum/pathology ; Case-Control Studies ; ROC Curve
    • Abstract:
      Objective: Neonatal necrotizing enterocolitis (NEC) is a serious intestinal inflammatory disease. We investigated intestinal fatty acid binding protein (I-FABP), I-FABP mRNA, and interleukin-6 (IL-6) as potential diagnostic biomarkers in NEC.
      Methods: Forty mice were subjected to hypoxic-ischemic intestinal injury, and then serum I-FABP protein and mRNA levels were quantified. Ileal tissue pathological scores were determined by hematoxylin and eosin staining. I-FABP expression levels and translocation in these tissues were detected using western blotting and immunofluorescence, respectively. Samples from 30 human neonates with NEC and 30 healthy neonates had serum I-FABP protein/mRNA and IL-6 levels measured.
      Results: The mouse ileal tissue pathological score and I-FABP levels, as well as serum I-FABP and I-FABP mRNA levels, were significantly higher in the model group than in the control group. Serum I-FABP, I-FABP mRNA, and IL-6 levels were significantly higher in human neonates with NEC than in the healthy group. Logistic regression and receiver operating curve analyses revealed that I-FABP protein/mRNA and IL-6 levels could be diagnostic biomarkers for NEC.
      Conclusions: I-FABP protein/mRNA and IL-6 levels are useful biomarkers of intestinal ischemic injury in neonates with NEC. The combined detection of I-FABP protein/mRNA and IL-6 is recommended rather than using a single biomarker.
      Competing Interests: Declaration of conflicting interestThe authors declare that there is no conflict of interest.
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    • Contributed Indexing:
      Keywords: Neonatal necrotizing enterocolitis; diagnostic biomarker; interleukin-6; intestinal disease; intestinal fatty acid binding protein; mouse model
    • Accession Number:
      0 (Fatty Acid-Binding Proteins)
      0 (Interleukin-6)
      0 (Biomarkers)
      0 (RNA, Messenger)
      0 (FABP2 protein, human)
    • Publication Date:
      Date Created: 20240613 Date Completed: 20240613 Latest Revision: 20240626
    • Publication Date:
      20240626
    • Accession Number:
      PMC11179468
    • Accession Number:
      10.1177/03000605241254788
    • Accession Number:
      38867509