Establishment of an in vitro cell coculture model for investigating the whitening mechanism of Paeonia lactiflora Pall seeds oil.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read Online Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Blackwell Science Country of Publication: England NLM ID: 101130964 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1473-2165 (Electronic) Linking ISSN: 14732130 NLM ISO Abbreviation: J Cosmet Dermatol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Oxford, UK : Blackwell Science, c2002-
    • Subject Terms:
      Paeonia*/chemistry ; Coculture Techniques* ; Melanins*/biosynthesis ; Seeds*/chemistry ; Plant Oils*/pharmacology ; Monophenol Monooxygenase*/metabolism ; Cell Proliferation*/drug effects ; Skin Lightening Preparations*/pharmacology; Humans ; Mice ; Animals ; HaCaT Cells
    • Abstract:
      Background: In vitro single-cell experiments may yield inconsistent results compared to clinical trials. To enhance the reliability of cosmetic active ingredient screening, a coculture model of B16F10-HaCaT cells was established in vitro based on the structural characteristics of human skin, thereby improving the credibility of experimental outcomes. Currently, most cosmetic whitening additives primarily target simple efficacy goals such as inhibiting tyrosinase activity or melanin transfer. Therefore, investigating novel and efficient whitening additives has become a prominent research focus.
      Objectives: The aim is to establish an in vitro cell coculture model for more reliable experimental results and investigate the mechanism by which Paeonia lactiflora Pall seeds oil inhibits melanin production and transfer.
      Methods: The impact of different concentrations of Paeonia lactiflora Pall seeds oil on cocultured cell proliferation rate was assessed using cck8 assay. Tyrosinase inhibition ability in cocultured cells was tested using levodopa as a substrate. Melanin production inhibition ability in coculture cells was evaluated by lysing cells with sodium hydroxide. The effect of Paeonia lactiflora Pall seeds oil on dendrite-related gene expression levels was examined through qPCR analysis. Additionally, Western blotting was employed to study the effect of Paeonia lactiflora Pall seeds oil on dendrite-related protein expression levels.
      Results: Different concentrations of Paeonia lactiflora Pall seeds oil did not affect the proliferation activity of cocultured cells. A specific concentration of α-MSH increased cell tyrosinase activity, cellular melanin content, as well as Rac1, Cdc42, and PAR-2 gene and protein expression related to dendritic formation. Treatment with a certain concentration of Paeonia lactiflora Pall seeds oil resulted in decreased tyrosinase activity and melanin content in cells along with downregulated expression levels of Rac1, Cdc42, and PAR-2 genes and proteins associated with dendritic formation.
      Conclusions: Paeonia lactiflora Pall seeds oil at specific concentrations exhibits the ability to inhibit tyrosinase activity, decrease melanin content, and possesses the potential to impede melanin transfer.
      (© 2024 The Author(s). Journal of Cosmetic Dermatology published by Wiley Periodicals LLC.)
    • References:
      He DY, Dai SM. Anti‐inflammatory and immunomodulatory effects of Paeonia lactiflora pall, a traditional Chinese herbal medicine. Front Pharmacol. 2011;2:9394.
      Nie R, Zhang Y, Zhang H, Jin Q, Wu G, Wang X. Effect of different processing methods on physicochemical properties, chemical compositions and in vitro antioxidant activities of Paeonia lactiflora pall seed oils. Food Chem. 2020;332:127408.
      Wu G, Shen Y, Nie R, et al. The bioactive compounds and cellular antioxidant activity of herbaceous peony (Paeonia lactiflora Pall) seed oil from China. J Food Sci. 2020;85(11):3815‐3822.
      Magid AA, Schmitt M, Prin PC, et al. In vitro tyrosinase inhibitory and antioxidant activities of extracts and constituents of Paeonia lactiflora Pall. Flowers. J Nat Prod. 2017;7(3):237‐245.
      Wen SY, Wu YS, Liu H, et al. Paeoniflorin found in Paeonia lactiflora root extract inhibits melanogenesis by regulating melanin‐related signal transduction in B16F10 cells. J Cosmet Dermatol. 2023;22(10):2824‐2830.
      Li AP. In vitro evaluation of human xenobiotic toxicity: scientific concepts and the novel integrated discrete multiple cell coculture (IdMOC) technology. ALTEX. 2008;25(1):43‐49.
      Thélu A, Catoire S, Kerdine‐Römer S. Immune‐competent in vitro co‐culture models as an approach for skin sensitisation assessment. Toxicol In Vitro. 2020;62:104691.
      Fajuyigbe D, Lwin SM, Diffey BL, et al. Melanin distribution in human epidermis affords localized protection against DNA photodamage and concurs with skin cancer incidence difference in extreme phototypes. FASEB J. 2018;32(7):3700‐3706.
      Chen T, Su WC, Wu YN, et al. Inhibitory effect of CADI on melanin transfer in the B16F10‐HaCAT cells co‐culture system and anti‐melanogenesis of CNCE in zebrafish. Process Biochem. 2021;105:50‐61.
      Luo ZX, Xiang LH, Liu GY. Effects of the herbal active ingredients Osthole and Baicalin on dendricity of melanocytes in co‐cultrue of human melanocytes and keratinoeytes. Chin J Derm Venereol. 2015;29(2):136‐139.
      Ha SY, Jung JY, Yang JK. Camellia japonica essential oil inhibits α‐MSH‐induced melanin production and tyrosinase activity in B16F10 melanoma cells. Evid Based Complement Alternat Med. 2021;2021:1‐8.
      Li Y, Huang J, Lu J, et al. The role and mechanism of Asian medicinal plants in treating skin pigmentary disorders. J Ethnopharmacol. 2019;245:112173.
      Lee CS, Nam G, Bae IH, Park J. Whitening efficacy of ginsenoside F1 through inhibition of melanin transfer in cocultured human melanocytes–keratinocytes and three‐dimensional human skin equivalent. J Ginseng Res. 2019;43(2):300‐304.
      Chen Z, Hong N, Yan C, Zheng Z, Xi J, Cao P. The potential of Paeonia lactiflora seeds oil as a pure natural cosmetics raw material: in vitro findings. J Cosmet Dermatol. 2024;23:1875‐1883.
      Hseu YC, Yeh JT, Vadivalagan C, et al. The in vitro and in vivo depigmentation activity of coenzyme Q0, a major quinone derivative from Antrodia camphorata, through autophagy induction in human melanocytes and keratinocytes. Cell Commun Signal. 2024;22(1):151.
      Lv K, Li J, Zhang D. Protection of human skin cells against stress damage by use of Y2SK2 and SK3 type dehydrins from agapanthus praecox. China Surfactant Deterg Cosmet. 2020;50(2):76‐85.
      Maresca V, Flori E, Bellei B, Aspite N, Kovacs D, Picardo M. MC1R stimulation by α‐MSH induces catalase and promotes its re‐distribution to the cell periphery and dendrites. Pigment Cell Melanoma Res. 2010;23(2):263‐275.
      Thody AJ. α‐MSH and the regulation of melanocyte function. Ann N Y Acad Sci. 1999;885(1):217‐229.
      Qian W, Liu W, Zhu D, et al. Natural skin‐whitening compounds for the treatment of melanogenesis. Exp Ther Med. 2020;20(1):173‐185.
      Schutte SC, Kadakia F, Davidson S. Skin‐nerve co‐culture systems for disease modeling and drug discovery. Tissue Eng Part C Methods. 2021;27(2):89‐99.
      Joshi PG, Nair N, Begum G, Joshi NB, Sinkar VP, Vora S. Melanocyte–keratinocyte interaction induces calcium signalling and melanin transfer to keratinocytes. Pigment Cell Res. 2007;20(5):380‐384.
      Kim KS, Kim JA, Eom SY, Lee SH, Min KR, Kim Y. Inhibitory effect of piperlonguminine on melanin production in melanoma B16 cell line by downregulation of tyrosinase expression. Pigment Cell Res. 2006;19(1):90‐98.
      Enkhtaivan E, Lee CH. Role of amine neurotransmitters and their receptors in skin pigmentation: therapeutic implication. Int J Mol Sci. 2021;22(15):8071.
      Scott G, Leopardi S, Parker L, et al. The proteinase ‐ activated receptor ‐ 2 mediates phagocytosis in a rho ‐ dependent manner in human keratinocytes. J Invest Dermatol. 2003;121(3):529‐541.
      Kino‐oka M, Kim J, Kurisaka K, Kim MH. Preferential growth of skeletal myoblasts and fibroblasts in co‐culture on a dendrimer‐immobilized surface. J Biosci Bioeng. 2013;115(1):96‐99.
      Etienne‐Manneville S, Hall A. Rho GTPases in cell biology. Nature. 2002;420(6916):629‐635.
      Scott GA, Cassidy L. Rac1 mediates dendrite formation in response to melanocyte stimulating hormone and ultraviolet light in a murine melanoma model. J Invest Dermatol. 1998;111(2):243‐250.
      Scott G, Leopardi S. The cAMP signaling pathway has opposing effects on Rac and rho in B16F10 cells: implications for dendrite formation in melanocytic cells. Pigment Cell Res. 2003;16(2):139‐148.
      Lv J, Yang Y, Jia B, Li S, Zhang X, Gao R. The inhibitory effect of curcumin derivative J147 on melanogenesis and melanosome transport by facilitating ERK‐mediated MITF degradation. Front Pharmacol. 2021;12:783730.
    • Contributed Indexing:
      Keywords: Paeonia lactiflora Pall; coculture; melanin content; melanin transfer
    • Accession Number:
      0 (Melanins)
      0 (Plant Oils)
      EC 1.14.18.1 (Monophenol Monooxygenase)
      0 (Skin Lightening Preparations)
    • Publication Date:
      Date Created: 20240612 Date Completed: 20240828 Latest Revision: 20240828
    • Publication Date:
      20240828
    • Accession Number:
      10.1111/jocd.16370
    • Accession Number:
      38864461