Heat shock protein 70 kDa (HSP70) is involved in the maintenance of pig sperm function throughout liquid storage at 17 °C.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

HSP70 Heat-Shock Proteins*/metabolism ; Spermatozoa*/metabolism ; Spermatozoa*/physiology ; Semen Preservation*/methods ; Semen Preservation*/veterinary ; Sperm Motility*/drug effects ; Reactive Oxygen Species*/metabolism; Animals ; Male ; Swine ; Mitochondria/metabolism ; Semen Analysis ; Cell Survival/drug effects ; Calcium/metabolism

At present, liquid storage is the most efficient method for pig semen preservation. This approach relies upon reducing sperm metabolism, allowing for the maintenance of cell lifespan. In this context, the study of proteins that could protect sperm during liquid storage is of high relevance. The 70 kDa Heat Shock Protein (HSP70) is an anti-apoptotic protein that has been reported to be relevant to sperm survival. Thus, we explored the role of HSP70 during prolonged storage of pig semen at 17 °C. Six semen pools were incubated with YM-1 (0, 0.05, 0.1 and 0.2 μM), an HSP70 inhibitor, and stored at 17 °C for 21 days. On days 0, 4, 10, 14 and 21, sperm quality and function were evaluated through flow cytometry and Computer-Assisted Sperm Analysis (CASA), and HSP70 activity and chromatin condensation were also determined. While inhibition of HSP70 increased progressive motility, Ca ²⁺ and Reactive Oxygen Species (ROS) levels, and mitochondrial activity during the first 10 days of storage, it had a detrimental effect on sperm motility after 14 and 21 days. In spite of this, sperm viability was not altered. We can conclude that HSP70 contributes to the liquid storage of pig semen because it keeps mitochondrial activity low, which is needed for the maintenance of sperm function.
(© 2024. The Author(s).)

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2021-SGR-00900 Agència de Gestió d'Ajuts Universitaris i de Recerca; PID2020-113320RB-I00 Ministerio de Ciencia e Innovación; ICREA Academia Institució Catalana de Recerca i Estudis Avançats

Keywords: Flow cytometry; HSP70; Liquid storage; Pig; Sperm; YM-1

0 (HSP70 Heat-Shock Proteins)
0 (Reactive Oxygen Species)
SY7Q814VUP (Calcium)

Date Created: 20240611 Date Completed: 20240611 Latest Revision: 20240625

20240625

PMC11166661

10.1038/s41598-024-64488-5

38862610