Alternative splicing of the conserved drug-resistant orthologue FpNcb2 is associated with its nuclear accumulation of products and full virulence of Fusarium pseudograminearum.

Publisher: Published for SCI by Wiley Country of Publication: England NLM ID: 100898744 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1526-4998 (Electronic) Linking ISSN: 1526498X NLM ISO Abbreviation: Pest Manag Sci Subsets: MEDLINE

Original Publication: West Sussex, UK : Published for SCI by Wiley, c2000-

Fusarium*/genetics ; Fusarium*/metabolism ; Alternative Splicing* ; Fungal Proteins*/genetics ; Fungal Proteins*/metabolism ; Drug Resistance, Fungal*/genetics; Virulence/genetics ; Plant Diseases/microbiology ; Cell Nucleus/metabolism ; Cell Nucleus/genetics ; Fungicides, Industrial/pharmacology ; Spores, Fungal/genetics

Background: Negative cofactor 2 NC2β (Ncb2 or Dr1) is the beta subunit of a conserved heterodimeric regulator of transcription negative cofactor 2 (NC2) complex that has been identified as key regulator of drug resistance in model fungi. However, its role in plant pathogens is still unclear.
Results: We identified an NC2β orthologue, FpNcb2, in Fusarium pseudograminearum, which is not only a significant regulatory function in drug resistance, but also essential for growth, conidiation and penetration. Moreover, FpNcb2 undergoes alternative splicing which creates two mRNA isoforms. As a putative CCAAT binding protein, FpNcb2 concentrates in the nuclei, contributing to the expression of two spliced mRNA of FpNcb2 in hypha, conidiophores and conidia, with exception of FpNcb2 ^ISOA in germlings. Expression of each spliced mRNA of FpNcb2 in Δfpncb2 mutant could full complement the defects on growth, conidiation and fungicides sensitivity to that of wild type. However, FpNcb2 ^ISOA and FpNcb2 ^ISOB have different effects on virulence. FpNcb2 acts as a regulator for the transcription of some genes encoding drug efflux and hydrolases.
Conclusion: Our analysis showed the existence of alternative mRNA splicing in the NC2β orthologue, which is associated with protein subcellular localization and fungal virulence. The further elucidation of the target genes of NC2β will provide insights into the potential regulation mechanisms in the antifungal resistance and pathogenesis of F. pseudograminearum. © 2024 Society of Chemical Industry.
(© 2024 Society of Chemical Industry.)

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Keywords: Fusarium pseudograminearum; NC2β; drug‐resistant gene; pathogenesis

0 (Fungal Proteins)
0 (Fungicides, Industrial)

Date Created: 20240611 Date Completed: 20241011 Latest Revision: 20241022

20241023

10.1002/ps.8219

38860488