Birch pollen-induced signatures in dendritic cells are maintained upon additional cytomegalovirus exposure.

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  • Additional Information
    • Source:
      Publisher: Elsevier/North-Holland Country of Publication: Netherlands NLM ID: 7706761 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0038 (Electronic) Linking ISSN: 03781119 NLM ISO Abbreviation: Gene Subsets: MEDLINE
    • Publication Information:
      Original Publication: Amsterdam, Elsevier/North-Holland, 1976-
    • Subject Terms:
      Dendritic Cells*/virology ; Dendritic Cells*/metabolism ; Dendritic Cells*/immunology ; Pollen*/genetics ; Pollen*/immunology ; Cytomegalovirus*/genetics ; Cytomegalovirus*/physiology ; Betula*; Humans ; Phosphatidylinositol 3-Kinases/metabolism ; Cytomegalovirus Infections/virology ; Cytomegalovirus Infections/genetics ; Cytomegalovirus Infections/immunology ; Transcriptome ; Signal Transduction ; Proto-Oncogene Proteins c-akt/metabolism ; Transcription Factor RelA/metabolism ; Transcription Factor RelA/genetics ; Cells, Cultured
    • Abstract:
      During the birch pollen season an enhanced incidence of virus infections is noticed, raising the question whether pollen can affect anti-viral responses independent of allergic reactions. We previously showed that birch pollen-treatment of monocyte-derived dendritic cells (moDC) enhances human cytomegalovirus (HCMV) infection. Here we addressed how in moDC the relatively weak pollen response can affect the comparably strong response to HCMV. To this end, moDC were stimulated with aqueous birch pollen extract (APE), HCMV, and APE with HCMV, and transcriptomic signatures were determined after 6 and 24 h of incubation. Infection was monitored upon exposure of moDC to GFP expressing HCMV by flow cytometric analysis of GFP expressing cells. Principle component analysis of RNA sequencing data revealed close clustering of mock and APE treated moDC, whereas HCMV as well as APE with HCMV treated moDC clustered separately after 6 and 24 h of incubation, respectively. Communally induced genes were detected in APE, HCMV and APE with HCMV treated moDC. In APE with HCMV treated moDC, the comparably weak APE induced signatures were maintained after HCMV exposure. In particular, NF-κB/RELA and PI3K/AKT/MAPK signaling were altered upon APE with HCMV exposure. Earlier, we discovered that NF-κB inhibition alleviated APE induced enhancement of HCMV infection. Here we additionally found that impairment of PI3K signaling reduced HCMV infection in HCMV and APE with HCMV treated moDC. APE treated moDC that were exposed to HCMV show a unique host gene signature, which to a large extent is regulated by NF-κB activation and PI3K/AKT/MAPK signaling.
      Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: Antiviral immunity; Aqueous birch pollen extract; Monocyte-derived dendritic cells; PI3K signaling; Pro-inflammatory cytokines; RNA-sequencing; Viral infection
    • Accession Number:
      EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
      EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
      0 (Transcription Factor RelA)
      0 (RELA protein, human)
    • Publication Date:
      Date Created: 20240609 Date Completed: 20240725 Latest Revision: 20240725
    • Publication Date:
      20240726
    • Accession Number:
      10.1016/j.gene.2024.148649
    • Accession Number:
      38852697