Aptamer responsive DNA Functionalized hydrogels electrochemiluminescence biosensor for the detection of adenosine triphosphate.

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  • Additional Information
    • Source:
      Publisher: Elsevier Advanced Technology Country of Publication: England NLM ID: 9001289 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4235 (Electronic) Linking ISSN: 09565663 NLM ISO Abbreviation: Biosens Bioelectron Subsets: MEDLINE
    • Publication Information:
      Publication: Oxford : Elsevier Advanced Technology
      Original Publication: [Barking, Essex, England] : Elsevier Applied Science, 1989-
    • Subject Terms:
    • Abstract:
      DNA hydrogel represents a noteworthy biomaterial. The preparation of biosensors by combining DNA hydrogel with electrochemiluminescence can simplify the modification process and raise the experimental efficiency. In this study, an electrochemiluminescence (ECL) biosensor based on DNA hydrogel was fabricated to detect adenosine triphosphate (ATP) simply and quickly. CdTe-Ru@SiO 2 nanospheres capable of ECL resonance energy transfer (RET) were synthesized and encapsulated CdTe-Ru@SiO 2 in the DNA hydrogel to provide strong and stable ECL signals. DNA hydrogel avoided the labeling of ECL signal molecules. The aptamer of ATP as the linker of the hydrogel for the specificity of ATP detection. The cross-linked structure of the aptamer and the polymer chains was opened by ATP, and then the decomposition of the DNA hydrogel initiated the escape of CdTe-Ru@SiO 2 to generate an ECL signal. The designed biosensor detected ATP without too much modification and complex experimental steps on the electrode surface, with good specificity and stability, and a wide linear range. The detection range was 10-5000 nM, and the detection limit was 6.68 nM (S/N = 3). The combination of DNA hydrogel and ECL biosensor provided a new way for clinical detection of ATP and other biomolecule.
      Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2024 Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: Adenosine triphosphate; DNA hydrogel; Electrochemiluminescence; Resonance energy transfer
    • Accession Number:
      8L70Q75FXE (Adenosine Triphosphate)
      0 (Aptamers, Nucleotide)
      0 (Hydrogels)
      9007-49-2 (DNA)
      7631-86-9 (Silicon Dioxide)
      NQA0O090ZJ (Tellurium)
      0 (Cadmium Compounds)
      STG188WO13 (cadmium telluride)
    • Publication Date:
      Date Created: 20240609 Date Completed: 20240630 Latest Revision: 20240630
    • Publication Date:
      20240701
    • Accession Number:
      10.1016/j.bios.2024.116476
    • Accession Number:
      38852325