Study on the interaction between agglutinin and chondroitin sulfate and dermatan sulfate using multiple methods.

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  • Author(s): Liu S;Liu S; Zhang X; Zhang X; Chen Y; Chen Y; Li Y; Li Y; Liu X; Liu X
  • Source:
    International journal of biological macromolecules [Int J Biol Macromol] 2024 Jun; Vol. 272 (Pt 1), pp. 132624. Date of Electronic Publication: 2024 Jun 04.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE
    • Publication Information:
      Publication: Amsterdam : Elsevier
      Original Publication: Guildford, Eng., IPC Science and Technology Press.
    • Subject Terms:
      Chondroitin Sulfates*/chemistry ; Dermatan Sulfate*/chemistry ; Dermatan Sulfate*/metabolism ; Molecular Docking Simulation* ; Plant Lectins*/chemistry ; Plant Lectins*/metabolism ; Protein Binding*; Surface Plasmon Resonance ; Agglutinins/chemistry ; Agglutinins/metabolism ; Circular Dichroism ; Electrophoresis, Capillary
    • Abstract:
      In this work, the interaction of chondroitin sulfate (CS) and dermatan sulfate (DS) with plant lectins was studied by affinity capillary electrophoresis (ACE), surface plasmon resonance (SPR) technology, molecular docking simulation, and circular dichroism spectroscopy. The ACE method was used for the first time to study the interaction of Ricinus Communis Agglutinin I (RCA I), Wisteria Floribunda Lectin (WFA), and Soybean Agglutinin (SBA) with CS and DS, and the results were in good agreement with those of the SPR method. The results of experiments indicate that RCA I has a strong binding affinity with CS, and the sulfated position does not affect the relationship, but the degree of sulfation can affect the combination of RCA I with CS to some extent. However, the binding affinity with DS is very weak. This study lays the foundation for developing more specialized analysis methods for CS and DS based on RCA I.
      Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2024 Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: Chondroitin sulfate; Dermatan sulfate; Interaction; Multiple methods; Plant lectins
    • Accession Number:
      9007-28-7 (Chondroitin Sulfates)
      24967-94-0 (Dermatan Sulfate)
      0 (Plant Lectins)
      0 (Agglutinins)
    • Publication Date:
      Date Created: 20240605 Date Completed: 20240614 Latest Revision: 20240614
    • Publication Date:
      20240615
    • Accession Number:
      10.1016/j.ijbiomac.2024.132624
    • Accession Number:
      38838594