3D-bioprinted GelMA/gelatin/amniotic membrane extract (AME) scaffold loaded with keratinocytes, fibroblasts, and endothelial cells for skin tissue engineering.

Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE

Original Publication: London : Nature Publishing Group, copyright 2011-

Keratinocytes*/drug effects ; Keratinocytes*/cytology ; Keratinocytes*/metabolism ; Gelatin*/chemistry ; Tissue Engineering*/methods ; Fibroblasts*/drug effects ; Fibroblasts*/metabolism ; Fibroblasts*/cytology ; Tissue Scaffolds*/chemistry ; Amnion*/cytology ; Amnion*/metabolism ; Amnion*/chemistry ; Bioprinting*/methods ; Human Umbilical Vein Endothelial Cells*; Humans ; Printing, Three-Dimensional ; Skin/metabolism ; Skin/cytology ; Methacrylates/chemistry ; Cell Survival/drug effects ; Endothelial Cells/metabolism ; Endothelial Cells/drug effects ; Endothelial Cells/cytology

Gelatin-methacryloyl (GelMA) is a highly adaptable biomaterial extensively utilized in skin regeneration applications. However, it is frequently imperative to enhance its physical and biological qualities by including supplementary substances in its composition. The purpose of this study was to fabricate and characterize a bi-layered GelMA-gelatin scaffold using 3D bioprinting. The upper section of the scaffold was encompassed with keratinocytes to simulate the epidermis, while the lower section included fibroblasts and HUVEC cells to mimic the dermis. A further step involved the addition of amniotic membrane extract (AME) to the scaffold in order to promote angiogenesis. The incorporation of gelatin into GelMA was found to enhance its stability and mechanical qualities. While the Alamar blue test demonstrated that a high concentration of GelMA (20%) resulted in a decrease in cell viability, the live/dead cell staining revealed that incorporation of AME increased the quantity of viable HUVECs. Further, gelatin upregulated the expression of KRT10 in keratinocytes and VIM in fibroblasts. Additionally, the histological staining results demonstrated the formation of well-defined skin layers and the creation of extracellular matrix (ECM) in GelMA/gelatin hydrogels during a 14-day culture period. Our study showed that a 3D-bioprinted composite scaffold comprising GelMA, gelatin, and AME can be used to regenerate skin tissues.
(© 2024. The Author(s).)

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TUMS.VCR.REC.1398.972 Tehran University of Medical Sciences and Health Services

Keywords: Amniotic membrane extract; GelMA; Gelatin; Skin regeneration

9000-70-8 (Gelatin)
0 (Methacrylates)
0 (gelatin methacryloyl)

Date Created: 20240603 Date Completed: 20240603 Latest Revision: 20240607

20240607

PMC11148016

10.1038/s41598-024-62926-y

38830883