An electrochemiluminescent dual-signal immunosensor based on a Ru(bpy) 3 2+ -doped silica nanoparticle/copper nanocluster composite for okadaic acid detection.

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  • Additional Information
    • Source:
      Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 7702639 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7072 (Electronic) Linking ISSN: 03088146 NLM ISO Abbreviation: Food Chem Subsets: MEDLINE
    • Publication Information:
      Publication: Barking : Elsevier Applied Science Publishers
      Original Publication: Barking, Eng., Applied Science Publishers.
    • Subject Terms:
      Copper*/chemistry ; Silicon Dioxide*/chemistry ; Electrochemical Techniques* ; Luminescent Measurements*/methods ; Luminescent Measurements*/instrumentation ; Okadaic Acid*/analysis; Nanoparticles/chemistry ; Animals ; Biosensing Techniques ; Limit of Detection ; Immunoassay/methods ; Immunoassay/instrumentation ; Metal Nanoparticles/chemistry
    • Abstract:
      In this study, a sensitive dual-signal electrochemiluminescence (ECL) immunosensor was developed for okadaic acid (OA) detection utilizing copper nanoclusters (CuNCs) and Ru(bpy) 3 2+ -doped silica nanoparticles (RuSiNPs). Interestingly, the CuNCs could simultaneously enhance both cathodic (-0.95 V) and anodic (+1.15 V) ECL signals of RuSiNPs, forming a dual-signal ECL sensing platform. Further, RuSiNPs@CuNCs were used as immunomarkers by covalently conjugating them with an anti-OA monoclonal antibody (mAb) to form probes. Finally, dual ECL signals of the immunosensor were fabricated and showed good linear relationships with OA concentrations in the range of 0.05-70 ng mL -1 , having a median inhibitory concentration (IC 50 ) of 1.972 ng mL -1 and a limit of detection of 0.039 ng mL -1 . Moreover, the constant ratio of the cathodic and anodic ECL peaks achieved self-calibration of the detection signal and improved the reliability of the results. Finally, we successfully applied the ECL sensor to detect OA in spiked oyster samples.
      Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2023. Published by Elsevier Ltd.)
    • Contributed Indexing:
      Keywords: Copper nanoclusters; Electrochemiluminescence; Immunosensor; Okadaic acid
    • Accession Number:
      789U1901C5 (Copper)
      7631-86-9 (Silicon Dioxide)
      1W21G5Q4N2 (Okadaic Acid)
    • Publication Date:
      Date Created: 20240601 Date Completed: 20240709 Latest Revision: 20240709
    • Publication Date:
      20240709
    • Accession Number:
      10.1016/j.foodchem.2024.139844
    • Accession Number:
      38823134