Antimicrobial resistance and genetic relatedness among Campylobacter coli and Campylobacter jejuni from humans and retail chicken meat in Taiwan.

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  • Additional Information
    • Source:
      Publisher: Published by Elsevier Ltd. on behalf of International Society of Chemotherapy for Infection and Cancer Country of Publication: Netherlands NLM ID: 101622459 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2213-7173 (Electronic) Linking ISSN: 22137165 NLM ISO Abbreviation: J Glob Antimicrob Resist Subsets: MEDLINE
    • Publication Information:
      Original Publication: Amsterdam : Published by Elsevier Ltd. on behalf of International Society of Chemotherapy for Infection and Cancer
    • Subject Terms:
      Campylobacter jejuni*/genetics ; Campylobacter jejuni*/drug effects ; Campylobacter jejuni*/isolation & purification ; Campylobacter coli*/drug effects ; Campylobacter coli*/genetics ; Campylobacter coli*/isolation & purification ; Chickens*/microbiology ; Multilocus Sequence Typing* ; Anti-Bacterial Agents*/pharmacology ; Campylobacter Infections*/microbiology ; Campylobacter Infections*/epidemiology ; Meat*/microbiology ; Microbial Sensitivity Tests* ; Whole Genome Sequencing* ; Drug Resistance, Multiple, Bacterial*/genetics; Animals ; Humans ; Taiwan ; Genotype ; Drug Resistance, Bacterial/genetics ; Food Microbiology
    • Abstract:
      Objectives: Campylobacter is a significant zoonotic pathogen primarily transmitted through poultry. Our study aimed to assess antimicrobial resistance and genetic relationships among Campylobacter isolates from retail chicken meat and humans in Taiwan.
      Methods: Campylobacter isolates were analysed using whole-genome sequencing to investigate their antimicrobial resistance, genetic determinants of resistance, and genotypes.
      Results: Campylobacter coli and Campylobacter jejuni accounted for 44.9% and 55.1% of chicken meat isolates, and 11.4% and 88.6% of human isolates, respectively. C. coli displayed significantly higher resistance levels. Furthermore, isolates from chicken meat exhibited higher levels of resistance to most tested antimicrobials compared to isolates from humans. Multidrug resistance was observed in 96.3% of C. coli and 43.3% of C. jejuni isolates from chicken meat and 80.6% of C. coli and 15.8% of C. jejuni isolates from humans. Macrolide resistance was observed in 85.5% of C. coli isolates, primarily attributed to the erm(B) rather than the A2075G mutation in 23S rRNA. Among the 511 genomes, we identified 133 conventional MLST sequence types, indicating significant diversity among Campylobacter strains. Notably, hierarchical Core-genome multilocus sequence typing clustering, including HC0, HC5, and HC10, revealed a significant proportion of closely related isolates from chicken meat and humans.
      Conclusions: Our research highlights significant associations in antimicrobial resistance and genetic relatedness between Campylobacter isolates from chicken meat and humans in Taiwan. The genetic analysis data suggest that campylobacteriosis outbreaks may occur more frequently in Taiwan than previously assumed. Our study emphasizes the need for strategies to control multidrug-resistant strains and enhance outbreak prevention.
      (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
    • Contributed Indexing:
      Keywords: Antimicrobial resistance (AMR); Antimicrobial resistance gene (ARG); Campylobacter; Core-genome multilocus sequence typing (cgMLST); Molecular epidemiology; Whole-genome sequencing (WGS)
    • Accession Number:
      0 (Anti-Bacterial Agents)
    • Publication Date:
      Date Created: 20240531 Date Completed: 20240912 Latest Revision: 20240912
    • Publication Date:
      20240913
    • Accession Number:
      10.1016/j.jgar.2024.05.013
    • Accession Number:
      38821444