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SPAG5 deficiency activates autophagy to reduce atherosclerotic plaque formation in ApoE -/- mice.
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- Author(s): Guo L;Guo L; Yuan H; Yuan H; Zhu H; Zhu H; Zhou J; Zhou J; Wan Z; Wan Z; Zhou Y; Zhou Y
- Source:
BMC cardiovascular disorders [BMC Cardiovasc Disord] 2024 May 28; Vol. 24 (1), pp. 275. Date of Electronic Publication: 2024 May 28.- Publication Type:
Journal Article- Language:
English - Source:
- Additional Information
- Source: Publisher: BioMed Central Country of Publication: England NLM ID: 100968539 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2261 (Electronic) Linking ISSN: 14712261 NLM ISO Abbreviation: BMC Cardiovasc Disord Subsets: MEDLINE
- Publication Information: Original Publication: London : BioMed Central, [2001-
- Subject Terms: Apoptosis*/drug effects ; Atherosclerosis*/genetics ; Atherosclerosis*/pathology ; Autophagy*/drug effects ; Autophagy*/genetics ; Plaque, Atherosclerotic*/genetics; Animals ; Humans ; Male ; Mice ; Aorta/pathology ; Aorta/metabolism ; Aortic Diseases/pathology ; Aortic Diseases/genetics ; Aortic Diseases/prevention & control ; Aortic Diseases/metabolism ; Apolipoproteins E/genetics ; Autophagy-Related Proteins/metabolism ; Autophagy-Related Proteins/genetics ; Cell Cycle Proteins/metabolism ; Cell Cycle Proteins/genetics ; Cell Proliferation/drug effects ; Cells, Cultured ; Disease Models, Animal ; Human Umbilical Vein Endothelial Cells/metabolism ; Human Umbilical Vein Endothelial Cells/pathology ; Lipoproteins, LDL/metabolism ; Mice, Inbred C57BL ; Mice, Knockout, ApoE ; Phosphatidylinositol 3-Kinase/metabolism ; Proto-Oncogene Proteins c-akt/metabolism ; Signal Transduction ; TOR Serine-Threonine Kinases/metabolism
- Abstract: Background: Autophagy, as a regulator of cell survival, plays an important role in atherosclerosis (AS). Sperm associated antigen 5 (SPAG5) is closely associated with the classical autophagy pathway, PI3K/Akt/mTOR signaling pathway. This work attempted to investigate whether SPAG5 can affect AS development by regulating autophagy.
Methods: Human umbilical vein endothelial cells (HUVECs) were treated with oxidized-low density lipoprotein (ox-LDL) to induce cell damage. ApoE -/- mice were fed a Western diet to establish an AS mouse model. Haematoxylin and eosin (H&E) staining and Oil Red O staining evaluated the pathological changes and in lipid deposition in aortic tissues. CCK-8 and flow cytometry detected cell proliferation and apoptosis. Immunohistochemistry, Enzyme linked immunosorbent assay, qRT-PCR and western blotting assessed the levels of mRNA and proteins.
Results: Ox-LDL treatment elevated SPAG5 expression and the expression of autophagy-related proteins, LC3-I, LC3-II, Beclin-1, and p62, in HUVECs. GFP-LC3 dots were increased in ox-LDL-treated HUVECs and LPS-treated HUVECs. SPAG5 knockdown reversed both ox-LDL and LPS treatment-mediated inhibition of cell proliferation and promotion of apoptosis in HUVECs. SPAG5 silencing further elevated autophagy and repressed the expression of PI3K, p-Akt/Akt, and p-mTOR/mTOR in ox-LDL-treated HUVECs. 3-MA (autophagy inhibitor) treatment reversed SPAG5 silencing-mediated increase of cell proliferation and decrease of apoptosis in ox-LDL-treated HUVECs. In vivo, SPAG5 knockdown reduced atherosclerotic plaques in AS mice through activating autophagy and inhibiting PI3K/Akt/mTOR signaling pathway.
Conclusion: This work demonstrated that SPAG5 knockdown alleviated AS development through activating autophagy. Thus, SPAG5 may be a potential target for AS therapy.
(© 2024. The Author(s).) - References: Biochem Biophys Res Commun. 2006 May 5;343(2):428-34. (PMID: 16546135)
Cardiovasc Pathol. 2013 Jan-Feb;22(1):9-15. (PMID: 22818581)
Autophagy. 2022 Oct;18(10):2270-2287. (PMID: 35090378)
Elife. 2021 Apr 26;10:. (PMID: 33899739)
Trends Cancer. 2020 Jul;6(7):543-547. (PMID: 32291236)
Pharmacol Res. 2021 Apr;166:105169. (PMID: 33053445)
Phytomedicine. 2019 May;58:152765. (PMID: 31005720)
J Exp Clin Cancer Res. 2018 Sep 18;37(1):229. (PMID: 30249289)
Mol Cancer. 2018 Aug 8;17(1):117. (PMID: 30089483)
Biomolecules. 2022 Dec 21;13(1):. (PMID: 36671400)
Am J Chin Med. 2021;49(8):1913-1927. (PMID: 34775933)
Cell Prolif. 2020 Feb;53(2):e12738. (PMID: 31957155)
Biomed Environ Sci. 2022 Jul 20;35(7):573-603. (PMID: 35945174)
Mol Reprod Dev. 2001 Aug;59(4):410-6. (PMID: 11468777)
Front Mol Biosci. 2021 Jun 25;8:695336. (PMID: 34250023)
J Cell Biochem. 2019 Jun;120(6):9291-9299. (PMID: 30548681)
Autophagy. 2021 Dec;17(12):4218-4230. (PMID: 33849389)
Theranostics. 2021 Mar 12;11(11):5404-5417. (PMID: 33859754)
Oncogene. 2018 Jul;37(29):3937-3952. (PMID: 29662193)
Microvasc Res. 2022 Jan;139:104252. (PMID: 34520772)
Cardiovasc Res. 2018 Mar 15;114(4):622-634. (PMID: 29360955)
Nature. 2021 Apr;592(7855):524-533. (PMID: 33883728)
Drug Dev Res. 2022 Aug;83(5):1125-1137. (PMID: 35417048)
Proc Natl Acad Sci U S A. 2001 Dec 4;98(25):14434-9. (PMID: 11724960)
Cell Death Differ. 2021 May;28(5):1493-1511. (PMID: 33230261)
Circ Res. 2022 Apr;130(7):1038-1055. (PMID: 35196865)
DNA Cell Biol. 2022 Oct;41(10):893-902. (PMID: 36264549)
Oxid Med Cell Longev. 2020 Sep 15;2020:5245308. (PMID: 33014272)
JAMA Cardiol. 2019 Mar 1;4(3):206-213. (PMID: 30758506)
Circ Res. 2016 Feb 19;118(4):620-36. (PMID: 26892962)
Int J Biochem Cell Biol. 2020 Sep;126:105797. (PMID: 32668328)
J Hematol Oncol. 2019 Feb 8;12(1):14. (PMID: 30736840)
J Am Coll Cardiol. 2022 Jul 19;80(3):219-229. (PMID: 35835495) - Grant Information: 20202BAB206039 Natural Science Foundation of Jiangxi Province; 202130442 the Science and Technology Plan Project of Jiangxi Health Commission
- Contributed Indexing: Keywords: Atherosclerosis; Autophagy; PI3K/Akt/mTOR; SPAG5
- Accession Number: 0 (Apoe protein, mouse)
0 (Apolipoproteins E)
0 (Autophagy-Related Proteins)
0 (Cell Cycle Proteins)
0 (Lipoproteins, LDL)
EC 2.7.1.1 (mTOR protein, mouse)
0 (oxidized low density lipoprotein)
EC 2.7.1.137 (Phosphatidylinositol 3-Kinase)
EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
EC 2.7.11.1 (TOR Serine-Threonine Kinases) - Publication Date: Date Created: 20240528 Date Completed: 20240529 Latest Revision: 20240822
- Publication Date: 20240823
- Accession Number: PMC11131316
- Accession Number: 10.1186/s12872-024-03945-5
- Accession Number: 38807081
- Source:
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