Decoding the effect of photoperiodic cues in transducing kisspeptin-melatonin circuit during the pubertal onset in common carp.

Publisher: Wiley-Liss Country of Publication: United States NLM ID: 8903333 Publication Model: Print Cited Medium: Internet ISSN: 1098-2795 (Electronic) Linking ISSN: 1040452X NLM ISO Abbreviation: Mol Reprod Dev Subsets: MEDLINE

Publication: <2005-> : Hoboken, N.J. : Wiley-Liss
Original Publication: New York, NY : A.R. Liss, 1988-

Melatonin*/metabolism ; Kisspeptins*/metabolism ; Kisspeptins*/genetics ; Photoperiod* ; Carps*/metabolism ; Carps*/genetics ; Carps*/growth & development ; Carps*/physiology ; Sexual Maturation*/physiology; Animals ; Female ; Fish Proteins/metabolism ; Fish Proteins/genetics

This study unravels the intricate interplay between photoperiod, melatonin, and kisspeptin to orchestrate the pubertal onset of Common carp. Female fingerlings exposed to long days (LD) exhibited a hormonal crescendo, with upregulated hypothalamic-pituitary-ovarian (HPO) axis genes (kiss1, kiss1r, kiss2, gnrh2, gnrh3) and their downstream targets (lhr, fshr, ar1, esr1). However, the expression of the melatonin receptor (mtnr1a) diminished in LD, suggesting a potential inhibitory role. This hormonal symphony was further amplified by increased activity of key transcriptional regulators (gata1, gata2, cdx1, sp1, n-myc, hoxc8, plc, tac3, tacr3) and decreased expression of delayed puberty genes (mkrn1, dlk1). In contrast, short days (SD) muted this hormonal chorus, with decreased gnrh gene and regulator expression, elevated mtnr1a, and suppressed gonadal development. In in-vitro, estradiol mimicked the LD effect, boosting gnrh and regulator genes while dampening mtnr1a and melatonin-responsive genes. Conversely, melatonin acted as a conductor, downregulating gnrh and regulator genes and amplifying mtnr1a. Our findings illuminate the crucial roles of melatonin and kisspeptin as opposing forces in regulating pubertal timing. LD-induced melatonin suppression allows the kisspeptin symphony to flourish, triggering GnRH release and, ultimately, gonadal maturation. This delicate dance between photoperiod, melatonin, and kisspeptin orchestrates common carp's transition from juvenile to reproductive life.
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CRG/2018/000992 SERB-DST, Government of India

Keywords: HPG axis; kisspeptin; melatonin; photoperiod; puberty

JL5DK93RCL (Melatonin)
0 (Kisspeptins)
0 (Fish Proteins)

Date Created: 20240527 Date Completed: 20240527 Latest Revision: 20241216

20241217

10.1002/mrd.23744

38800960