Brassinosteroids biosynthetic gene MdBR6OX2 regulates salt stress tolerance in both apple and Arabidopsis.

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  • Additional Information
    • Source:
      Publisher: Elsevier Science Country of Publication: France NLM ID: 9882449 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2690 (Electronic) Linking ISSN: 09819428 NLM ISO Abbreviation: Plant Physiol Biochem Subsets: MEDLINE
    • Publication Information:
      Publication: Amsterdam : Elsevier Science
      Original Publication: Paris : Gauthier-Villars, c1987-
    • Subject Terms:
      Malus*/genetics ; Malus*/metabolism ; Malus*/drug effects ; Brassinosteroids*/metabolism ; Brassinosteroids*/biosynthesis ; Brassinosteroids*/pharmacology ; Arabidopsis*/genetics ; Arabidopsis*/metabolism ; Arabidopsis*/drug effects ; Salt Tolerance*/genetics ; Gene Expression Regulation, Plant*/drug effects ; Plant Proteins*/genetics ; Plant Proteins*/metabolism; Plants, Genetically Modified ; Salt Stress/genetics ; Cytochrome P-450 Enzyme System/genetics ; Cytochrome P-450 Enzyme System/metabolism
    • Abstract:
      Salt stress is a critical limiting factor for fruit yield and quality of apples. Brassinosteroids (BRs) play an important role in response to abiotic stresses. In the present study, application of 2,4- Epicastasterone on seedlings of Malus 'M9T337' and Malus domestica 'Gala3' alleviated the physiological effects, such as growth inhibition and leaf yellowing, induced by salt stress. Further analysis revealed that treatment with NaCl induced expression of genes involved in BR biosynthesis in 'M9T337' and 'Gala3'. Among which, the expression of BR biosynthetic gene MdBR6OX2 showed a three-fold upregulation upon salt treatment, suggesting its potential role in response to salt stress in apple. MdBR6OX2, belonging to the CYP450 family, contains a signal peptide region and a P450 domain. Expression patterns analysis showed that the expression of MdBR6OX2 can be significantly induced by different abiotic stresses. Overexpressing MdBR6OX2 enhanced the tolerance of apple callis to salt stress, and the contents of endogenous BR-related compounds, such as Typhastero (TY), Castasterone (CS) and Brassinolide (BL) were significantly increased in transgenic calli compared with that of wild-type. Extopic expression of MdBR6OX2 enhanced tolerance to salt stress in Arabidopsis. Genes associated with salt stress were significantly up-regulated, and the contents of BR-related compounds were significantly elevated under salt stress. Our data revealed that BR-biosynthetic gene MdBR6OX2 positively regulates salt stress tolerance in both apple calli and Arabidopsis.
      Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2024. Published by Elsevier Masson SAS.)
    • Contributed Indexing:
      Keywords: Apple; Brassinosteroids; Hormone synthesis; MdBR6OX2; Salt stress
    • Accession Number:
      0 (Brassinosteroids)
      0 (Plant Proteins)
      9035-51-2 (Cytochrome P-450 Enzyme System)
    • Publication Date:
      Date Created: 20240526 Date Completed: 20240615 Latest Revision: 20240615
    • Publication Date:
      20240616
    • Accession Number:
      10.1016/j.plaphy.2024.108767
    • Accession Number:
      38797009