The MAP kinase FvHog1 regulates FB1 synthesis and Ca 2+ homeostasis in Fusarium verticillioides.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9422688 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3336 (Electronic) Linking ISSN: 03043894 NLM ISO Abbreviation: J Hazard Mater Subsets: MEDLINE
    • Publication Information:
      Original Publication: Amsterdam : Elsevier,
    • Subject Terms:
      Fusarium*/metabolism ; Fusarium*/genetics ; Homeostasis* ; Calcium*/metabolism ; Fumonisins*/metabolism ; Mitogen-Activated Protein Kinases*/metabolism ; Mitogen-Activated Protein Kinases*/genetics ; Fungal Proteins*/metabolism ; Fungal Proteins*/genetics; Phosphorylation ; Gene Expression Regulation, Fungal
    • Abstract:
      The high osmolarity glycerol 1 mitogen-activated protein kinase (Hog1-MAPK) cascade genes are important for diverse biological processes. The activated Hog1 upon multiple environmental stress stimuli enters into the nucleus where it directly phosphorylates transcription factors to regulate various physiological processes in phytopathogenic fungi. However, their roles have not been well-characterized in Fusarium verticillioides. In this study, FvHog1 is identified and functionally analyzed. The findings reveal that the phosphorylation level and nuclear localization of FvHog1 are increased in Fumonisin B1 (FB1)-inducing condition to regulate the expression of FB1 biosynthesis FUM genes. More importantly, the deletion mutants of Hog1-MAPK pathway show increased sensitivity to Ca 2+ stress and elevated intracellular Ca 2+ content. The phosphorylation level and nuclear localization of FvHog1 are increased with Ca 2+ treatment. Furthermore, our results show that FvHog1 can directly phosphorylate Ca 2+ -responsive zinc finger transcription factor 1 (FvCrz1) to regulate Ca 2+ homeostasis. In conclusion, our findings indicate that FvHog1 is required for FB1 biosynthesis, pathogenicity and Ca 2+ homeostasis in F. verticillioides. It provides a theoretical basis for effective prevention and control maize ear and stalk rot disease.
      Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2024 Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: Ca(2+) homeostasis; FB1; Fusarium verticillioides; Hog1-MAPK pathway; Pathogenicity
    • Accession Number:
      SY7Q814VUP (Calcium)
      0 (Fumonisins)
      EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
      0 (Fungal Proteins)
      3ZZM97XZ32 (fumonisin B1)
    • Subject Terms:
      Fusarium verticillioides
    • Publication Date:
      Date Created: 20240525 Date Completed: 20240611 Latest Revision: 20240621
    • Publication Date:
      20240621
    • Accession Number:
      10.1016/j.jhazmat.2024.134682
    • Accession Number:
      38795487