Multidrug-resistant Escherichia coli causing canine pyometra and urinary tract infections are genetically related but distinct from those causing prostatic abscesses.

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  • Additional Information
    • Source:
      Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
    • Publication Information:
      Original Publication: London : Nature Publishing Group, copyright 2011-
    • Subject Terms:
      Urinary Tract Infections*/microbiology ; Urinary Tract Infections*/veterinary ; Drug Resistance, Multiple, Bacterial*/genetics ; Dog Diseases*/microbiology ; Escherichia coli Infections*/microbiology ; Escherichia coli Infections*/veterinary ; Pyometra*/microbiology ; Pyometra*/veterinary ; Pyometra*/genetics ; Abscess*/microbiology ; Abscess*/veterinary; Dogs ; Animals ; Male ; Cats ; Female ; Cat Diseases/microbiology ; Uropathogenic Escherichia coli/genetics ; Uropathogenic Escherichia coli/drug effects ; Uropathogenic Escherichia coli/pathogenicity ; Escherichia coli/genetics ; Escherichia coli/pathogenicity ; Escherichia coli/drug effects ; Anti-Bacterial Agents/pharmacology ; Prostatic Diseases/microbiology ; Prostatic Diseases/veterinary ; Prostatic Diseases/genetics ; Virulence/genetics ; Virulence Factors/genetics
    • Abstract:
      Despite extensive characterisation of uropathogenic Escherichia coli (UPEC) causing urinary tract infections (UTIs), the genetic background of non-urinary extraintestinal pathogenic E. coli (ExPEC) in companion animals remains inadequately understood. In this study, we characterised virulence traits of 104 E. coli isolated from canine pyometra (n = 61) and prostatic abscesses (PAs) (n = 38), and bloodstream infections (BSIs) in dogs (n = 2), and cats (n = 3). A stronger association with UPEC of pyometra strains in comparison to PA strains was revealed. Notably, 44 isolates exhibited resistance to third-generation cephalosporins and/or fluoroquinolones, 15 were extended-spectrum ß-lactamase-producers. Twelve multidrug-resistant (MDR) strains, isolated from pyometra (n = 4), PAs (n = 5), and BSIs (n = 3), along with 7 previously characterised UPEC strains from dogs and cats, were sequenced. Genomic characteristics revealed that MDR E. coli associated with UTIs, pyometra, and BSIs belonged to international high-risk E. coli clones, including sequence type (ST) 38, ST131, ST617, ST648, and ST1193. However, PA strains belonged to distinct lineages, including ST12, ST44, ST457, ST744, and ST13037. The coreSNPs, cgMLST, and pan-genome illustrated intra-clonal variations within the same ST from different sources. The high-risk ST131 and ST1193 (phylogroup B2) contained high numbers of ExPEC virulence genes on pathogenicity islands, predominating in pyometra and UTI. Hybrid MDR/virulence IncF multi-replicon plasmids, containing aerobactin genes, were commonly found in non-B2 phylogroups from all sources. These findings offer genomic insights into non-urinary ExPEC, highlighting its potential for invasive infections in pets beyond UTIs, particularly with regards to high-risk global clones.
      (© 2024. The Author(s).)
    • References:
      Microbiol Spectr. 2016 Feb;4(1):. (PMID: 26999393)
      Microbiol Spectr. 2022 Dec 21;10(6):e0255422. (PMID: 36409140)
      Vet Microbiol. 2013 Oct 25;166(3-4):590-4. (PMID: 23953028)
      Genome Res. 2018 Sep;28(9):1395-1404. (PMID: 30049790)
      Adv Microb Physiol. 2014;65:337-72. (PMID: 25476769)
      Front Microbiol. 2020 Sep 25;11:2065. (PMID: 33101219)
      J Antimicrob Chemother. 2017 Aug 1;72(8):2145-2155. (PMID: 28541467)
      Pathogens. 2021 Jan 20;10(2):. (PMID: 33498288)
      Microbiol Mol Biol Rev. 2009 Dec;73(4):750-74. (PMID: 19946140)
      Bioinformatics. 2015 Nov 15;31(22):3691-3. (PMID: 26198102)
      Environ Microbiol Rep. 2013 Feb;5(1):58-65. (PMID: 23757131)
      Int J Med Microbiol. 2007 Jun;297(3):163-76. (PMID: 17374506)
      Nucleic Acids Res. 2017 Jul 3;45(W1):W30-W35. (PMID: 28472413)
      Bioinformatics. 2014 Aug 1;30(15):2114-20. (PMID: 24695404)
      Antimicrob Agents Chemother. 2022 Jul 19;66(7):e0051122. (PMID: 35658504)
      Infect Genet Evol. 2020 Nov;85:104453. (PMID: 32649994)
      Bioinformatics. 2014 May 1;30(9):1312-3. (PMID: 24451623)
      Int J Mol Sci. 2023 Jul 14;24(14):. (PMID: 37511208)
      J Glob Antimicrob Resist. 2019 Jun;17:53-57. (PMID: 30445208)
      Microb Genom. 2016 Apr 29;2(4):e000056. (PMID: 28348851)
      Comp Immunol Microbiol Infect Dis. 2012 Sep;35(5):461-7. (PMID: 22554919)
      Microorganisms. 2020 Oct 31;8(11):. (PMID: 33142871)
      Infect Immun. 2012 Dec;80(12):4115-22. (PMID: 22966046)
      Nat Rev Microbiol. 2018 Aug;16(8):484-495. (PMID: 29855597)
      Nucleic Acids Res. 1991 Dec 25;19(24):6823-31. (PMID: 1762913)
      Antimicrob Agents Chemother. 2014 Sep;58(9):4997-5004. (PMID: 24867985)
      Gut. 1998 Jan;42(1):29-35. (PMID: 9505882)
      PLoS Comput Biol. 2017 Jun 8;13(6):e1005595. (PMID: 28594827)
      Sci Rep. 2019 Sep 13;9(1):13245. (PMID: 31519972)
      J Antimicrob Chemother. 2017 Oct 1;72(10):2764-2768. (PMID: 29091202)
      Vet J. 2019 May;247:8-25. (PMID: 30971357)
      J Clin Microbiol. 2015 Aug;53(8):2410-26. (PMID: 25972421)
      Transbound Emerg Dis. 2022 Nov;69(6):3256-3273. (PMID: 35945191)
      Nucleic Acids Res. 2022 Jan 7;50(D1):D912-D917. (PMID: 34850947)
      Antibiotics (Basel). 2021 Nov 10;10(11):. (PMID: 34827312)
      Microb Drug Resist. 2019 Oct;25(8):1182-1190. (PMID: 31140920)
      Vet Microbiol. 2022 Aug;271:109493. (PMID: 35728389)
      Sci Rep. 2021 Jun 16;11(1):12728. (PMID: 34135355)
      Curr Microbiol. 2020 Nov;77(11):3361-3370. (PMID: 32910213)
      Microbes Infect. 2003 Apr;5(5):449-56. (PMID: 12738001)
      Infect Immun. 2011 Mar;79(3):1225-35. (PMID: 21220482)
      Front Microbiol. 2021 Oct 27;12:754931. (PMID: 34777312)
      Antimicrob Agents Chemother. 2014 Jul;58(7):3895-903. (PMID: 24777092)
      Theriogenology. 2008 Aug;70(3):375-83. (PMID: 18514299)
      Mol Microbiol. 2006 Jun;60(5):1136-51. (PMID: 16689791)
      Open Forum Infect Dis. 2015 Jun 06;2(3):ofv083. (PMID: 26199950)
      Int J Med Microbiol. 2006 Dec;296(8):513-20. (PMID: 17008127)
      J Infect Dis. 2000 May;181(5):1753-4. (PMID: 10823778)
      J Infect Dis. 2000 Jan;181(1):261-72. (PMID: 10608775)
      BMC Bioinformatics. 2009 Dec 15;10:421. (PMID: 20003500)
    • Grant Information:
      CU_FRB65_hea (90)_195_31_14 Thailand Science Research and Innovation; N42A660897 National Research Council of Thailand
    • Contributed Indexing:
      Keywords: Escherichia coli; Dogs; Multidrug resistance; Prostatic abscesses; Pyometra; Virulence
    • Accession Number:
      0 (Anti-Bacterial Agents)
      0 (Virulence Factors)
    • Publication Date:
      Date Created: 20240523 Date Completed: 20240523 Latest Revision: 20240526
    • Publication Date:
      20240526
    • Accession Number:
      PMC11116460
    • Accession Number:
      10.1038/s41598-024-62028-9
    • Accession Number:
      38782931