Genetic relatedness, antibiotic resistance, and virulence of Klebsiella pneumoniae isolated from health care and food sources in Wuhan, China.

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  • Additional Information
    • Source:
      Publisher: Mosby Country of Publication: United States NLM ID: 8004854 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1527-3296 (Electronic) Linking ISSN: 01966553 NLM ISO Abbreviation: Am J Infect Control Subsets: MEDLINE
    • Publication Information:
      Original Publication: St. Louis, Mosby
    • Subject Terms:
      Klebsiella pneumoniae*/drug effects ; Klebsiella pneumoniae*/genetics ; Klebsiella pneumoniae*/isolation & purification ; Microbial Sensitivity Tests* ; Klebsiella Infections*/microbiology ; Klebsiella Infections*/epidemiology ; Food Microbiology* ; Anti-Bacterial Agents*/pharmacology ; Biofilms*/growth & development ; Electrophoresis, Gel, Pulsed-Field* ; Drug Resistance, Bacterial*/genetics; China/epidemiology ; Humans ; Molecular Typing ; Virulence/genetics ; Genotype ; Molecular Epidemiology ; Meat/microbiology ; Polymerase Chain Reaction
    • Abstract:
      Background: To investigate genetic relatedness and antibiotic resistance of Klebsiella pneumoniae from retail meat samples, clinical source samples, and hospital environmental samples in Wuhan, China.
      Methods: Hypermucoviscosity and biofilm formation of K. pneumoniae were assessed by string test and crystal violet staining. MICs of 18 antimicrobials were determined by broth microdilution. PCR detected 14 antibiotic resistance genes. Genetic relatedness and clonal dissemination were analyzed by PFGE.
      Results: Among 5,730 samples, 46 were tested positive for K pneumoniae, with higher rates observed in meat (23.4%) than in clinical samples (0.6%) and hospital environmental samples (8.0%). Meat-derived isolates showed high resistance to tetracycline (36.4%, 4/11), sulfonamide (27.3%, 3/11), and gentamicin (27.3%, 3/11), whereas clinical isolates exhibited significant resistance to ampicillin-sulbactam (32.3%, 10/31). Multidrug resistance was observed in 17.4% (8/46) of the isolates, particularly in hospital environmental samples (3/4). Biofilm production was observed in 88.1% (37/42) of K pneumoniae. Pulsed-field gel electrophoresis analysis revealed patient-to-patient K pneumoniae transmission, transmission between patients and hospital environment, as well as cross-contamination between markets.
      Conclusions: The findings underscore the importance of comprehensive surveillance, infection control, and judicious antibiotic use in mitigating the impact of K pneumoniae on public health, especially in the food chain and health care settings.
      (Copyright © 2024 Association for Professionals in Infection Control and Epidemiology, Inc. Published by Elsevier Inc. All rights reserved.)
    • Contributed Indexing:
      Keywords: Foodborne infection; PFGE; Resistance profile
    • Accession Number:
      0 (Anti-Bacterial Agents)
    • Publication Date:
      Date Created: 20240523 Date Completed: 20240816 Latest Revision: 20240816
    • Publication Date:
      20240817
    • Accession Number:
      10.1016/j.ajic.2024.05.011
    • Accession Number:
      38782211