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Wando Mount Pleasant Library
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Pan-viral Antibody Repertoire of Aqueous Humor in Cytomegalovirus Uveitis.
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- Author(s): Tiu CK;Tiu CK;Tiu CK; Chia WN; Chia WN; Anderson DE; Anderson DE; Anderson DE; Chee SP; Chee SP; Chee SP; Chee SP; Chee SP; Chee SP; Wang LF; Wang LF; Wang LF; Siak J; Siak J; Siak J
- Source:
American journal of ophthalmology [Am J Ophthalmol] 2024 May 21; Vol. 266, pp. 218-226. Date of Electronic Publication: 2024 May 21.- Publication Type:
Journal Article- Language:
English - Source:
- Additional Information
- Publication Information: Ahead of Print
- Source: Publisher: Elsevier Science Country of Publication: United States NLM ID: 0370500 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1891 (Electronic) Linking ISSN: 00029394 NLM ISO Abbreviation: Am J Ophthalmol Subsets: MEDLINE
- Publication Information: Publication: 1999- : New York, NY : Elsevier Science
Original Publication: [Chicago, etc., Ophthalmic Pub. Co., etc.] - Abstract: Purpose: The identification of infectious etiologies is important in the management of uveitis. Ocular fluid testing is required, but multiplex testing faces challenges due to the limited volume sampled. The determination of antibody repertoire of aqueous humor (AH) is not possible with conventional assays. We investigated the use of a highly multiplexable serological assay VirScan, a Phage ImmunoPrecipitation Sequencing (PhIP-Seq) library derived from the sequences of more than 200 viruses to determine the antibody composition of AH in patients with uveitis.
Design: Prospective, case control study.
Methods: We analyzed the paired AH and plasma samples of 11 immunocompetent patients with active polymerase chain reaction-positive cytomegalovirus (CMV) anterior uveitis and the AH of 34 control patients undergoing cataract surgery with no known uveitis in an institutional practice. The samples were tested using VirScan PhIP-Seq, and the entire pan-viral antibody repertoire was determined using peptide tile ranking by normalized counts to identify significant antibodies enrichment against all viruses with human tropism.
Results: Significant enrichment of antibodies to Herpesviridae, Picornavirdae, and Paramyxoviridae was detectable in 20 µL of AH samples from patients with CMV uveitis and controls. Patients with CMV uveitis had relative enrichment of anti-CMV antibodies in AH compared with their plasma. Epitope-level mapping identified significant enrichment of antibodies against CMV tegument protein pp150 (P = 1.5e-06) and envelope glycoprotein B (P = .0045) in the AH compared with controls.
Conclusions: Our proof-of-concept study not only sheds light on the antibody repertoire of AH but also expands the utility of PhIP-Seq to future studies to detect antibodies in AH in the study of inflammatory eye diseases.
(Copyright © 2024 Elsevier Inc. All rights reserved.) - Publication Date: Date Created: 20240522 Latest Revision: 20240706
- Publication Date: 20240707
- Accession Number: 10.1016/j.ajo.2024.05.004
- Accession Number: 38777101
- Publication Information:
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