Potato E3 ubiquitin ligase StRFP1 positively regulates late blight resistance by degrading sugar transporters StSWEET10c and StSWEET11.

Publisher: Wiley on behalf of New Phytologist Trust Country of Publication: England NLM ID: 9882884 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-8137 (Electronic) Linking ISSN: 0028646X NLM ISO Abbreviation: New Phytol Subsets: MEDLINE

Publication: Oxford : Wiley on behalf of New Phytologist Trust
Original Publication: London, New York [etc.] Academic Press.

Plant Diseases*/microbiology ; Disease Resistance*/genetics ; Phytophthora infestans*/pathogenicity ; Solanum tuberosum*/microbiology ; Solanum tuberosum*/genetics ; Solanum tuberosum*/metabolism ; Plant Proteins*/metabolism ; Plant Proteins*/genetics ; Ubiquitin-Protein Ligases*/metabolism ; Ubiquitin-Protein Ligases*/genetics; Cell Membrane/metabolism ; Ubiquitination ; Gene Expression Regulation, Plant ; Sucrose/metabolism ; Plant Leaves/metabolism ; Plant Leaves/microbiology ; Protein Binding ; Protein Transport

Potato (Solanum tuberosum) is the fourth largest food crop in the world. Late blight, caused by oomycete Phytophthora infestans, is the most devastating disease threatening potato production. Previous research has shown that StRFP1, a potato Arabidopsis Tóxicos en Levadura (ATL) family protein, positively regulates late blight resistance via its E3 ligase activity. However, the underlying mechanism is unknown. Here, we reveal that StRFP1 is associated with the plasma membrane (PM) and undergoes constitutive endocytic trafficking. Its PM localization is essential for inhibiting P. infestans colonization. Through in vivo and in vitro assays, we investigated that StRFP1 interacts with two sugar transporters StSWEET10c and StSWEET11 at the PM. Overexpression (OE) of StSWEET10c or StSWEET11 enhances P. infestans colonization. Both StSWEET10c and StSWEET11 exhibit sucrose transport ability in yeast, and OE of StSWEET10c leads to an increased sucrose content in the apoplastic fluid of potato leaves. StRFP1 ubiquitinates StSWEET10c and StSWEET11 to promote their degradation. We illustrate a novel mechanism by which a potato ATL protein enhances disease resistance by degrading susceptibility (S) factors, such as Sugars Will Eventually be Exported Transporters (SWEETs). This offers a potential strategy for improving disease resistance by utilizing host positive immune regulators to neutralize S factors.
(© 2024 The Authors. New Phytologist © 2024 New Phytologist Foundation.)

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31761143007 National Natural Science Foundation of China; 32072121 National Natural Science Foundation of China; 32372172 National Natural Science Foundation of China; 2023YFF000404 National Key Research and Development Program of China

Keywords: E3 ligase; SWEETs; StRFP1; S factor; late blight; potato

0 (Plant Proteins)
EC 2.3.2.27 (Ubiquitin-Protein Ligases)
57-50-1 (Sucrose)

Date Created: 20240521 Date Completed: 20240620 Latest Revision: 20240720

20240720

10.1111/nph.19848

38769723