Developmental validation of the ForenSeq® Kintelligence kit, MiSeq FGx® sequencing system and ForenSeq Universal Analysis Software.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101317016 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-0326 (Electronic) Linking ISSN: 18724973 NLM ISO Abbreviation: Forensic Sci Int Genet Subsets: MEDLINE
    • Publication Information:
      Original Publication: Amsterdam : Elsevier
    • Subject Terms:
      Software* ; High-Throughput Nucleotide Sequencing* ; DNA Fingerprinting* ; Polymorphism, Single Nucleotide* ; Sequence Analysis, DNA*; Humans
    • Abstract:
      Forensic Investigative Genetic Genealogy, a recent sub discipline of forensic genomics, leverages the high throughput and sensitivity of detection of next generation sequencing and established genetic and genealogical approaches to support the identification of human remains from missing persons investigations and investigative lead generation in violent crimes. To facilitate forensic DNA evidence analysis, the ForenSeq® Kintelligence multiplex, consisting of 10,230 SNPs, was developed. Design of the ForenSeq Kintelligence Kit, the MiSeq FGx® Sequencing System and the ForenSeq Universal Analysis Software is described. Developmental validation in accordance with SWGDAM guidelines and forensic quality assurance standards, using single source samples, is reported for the end-to-end workflow from library preparation to data interpretation. Performance metrics support the conclusion that more genetic information can be obtained from challenging samples compared to other commercially available forensic targeted DNA assays developed for capillary electrophoresis (CE) or other current next generation sequencing (NGS) kits due to the higher number of markers, the overall shorter amplicon sizes (97.8% <150 bp), and kit design. Data indicate that the multiplex is robust and fit for purpose for a wide range of quantity and quality samples. The ForenSeq Kintelligence Kit and the Universal Analysis Software allow transfer of the genetic component of forensic investigative genetic genealogy to the operational forensic laboratory.
      Competing Interests: Declaration of Competing Interest All work was performed at Verogen, now a QIAGEN company, where the ForenSeq Kintelligence Kit and Universal Analysis Software were developed. All authors, except BB who is a consultant to Verogen, were employees at Verogen during the time the work was completed and declare no other competing interests.
      (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: ForenSeq kintelligence kit; ForenSeq universal analysis software; Forensic investigative genetic genealogy (FIGG); Massively parallel sequencing (MPS); MiSeq FGx sequencing system; Next generation sequencing (NGS); SWGDAM developmental validation; Single nucleotide polymorphisms (SNP)
    • Publication Date:
      Date Created: 20240519 Date Completed: 20240616 Latest Revision: 20240616
    • Publication Date:
      20240617
    • Accession Number:
      10.1016/j.fsigen.2024.103055
    • Accession Number:
      38762965