Abstract: The main challenge in the development of agrochemicals is the lack of new leads and/or targets. It is critical to discover new molecular targets and their corresponding ligands. YZK-C22 , which contains a 1,2,3-thiadiazol-[1,2,4]triazolo[3,4- b ][1,3,4]thiadiazole skeleton, is a fungicide lead compound with broad-spectrum fungicidal activity. Previous studies suggested that the [1,2,4]triazolo[3,4- b ][1,3,4]thiadiazole scaffold exhibited good antifungal activity. Inspired by this, a series of pyrrolo[2,3- d ]thiazole derivatives were designed and synthesized through a bioisosteric strategy. Compounds C1 , C9 , and C20 were found to be more active against Rhizoctonia solani than the positive control YZK-C22. More than half of the target compounds provided favorable activity against Botrytis cinerea , where the EC 50 values of compounds C4 , C6 , C8 , C10 , and C20 varied from 1.17 to 1.77 μg/mL. Surface plasmon resonance and molecular docking suggested that in vitro potent compounds C9 and C20 have a new mode of action instead of acting as pyruvate kinase inhibitors. Transcriptome analysis revealed that compound C20 can impact the tryptophan metabolic pathway, cutin, suberin, and wax biosynthesis of B. cinerea . Overall, pyrrolo[2,3- d ]thiazole is discovered as a new fungicidal lead structure with a potential new mode of action for further exploration.
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