A bimetallic peroxidase-mimicking nanozyme with antifouling property for construction of sensor array to identify phosphoproteins and diagnose cancers.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Elsevier Advanced Technology Country of Publication: England NLM ID: 9001289 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4235 (Electronic) Linking ISSN: 09565663 NLM ISO Abbreviation: Biosens Bioelectron Subsets: MEDLINE
    • Publication Information:
      Publication: Oxford : Elsevier Advanced Technology
      Original Publication: [Barking, Essex, England] : Elsevier Applied Science, 1989-
    • Subject Terms:
      Biosensing Techniques*/methods ; Phosphoproteins*/chemistry ; Phosphoproteins*/metabolism ; Neoplasms* ; Hydrogen Peroxide*/chemistry ; Zirconium*/chemistry; Humans ; Peroxidase/chemistry ; Dopamine/chemistry ; Limit of Detection ; Biomimetic Materials/chemistry ; Catalysis
    • Abstract:
      Protein phosphorylation is a significant post-translational modification that plays a decisive role in the occurrence and development of diseases. However, the rapid and accurate identification of phosphoproteins remains challenging. Herein, a high-throughput sensor array has been constructed based on a magnetic bimetallic nanozyme (Fe 3 O 4 @ZNP@UiO-66) for the identification and discrimination of phosphoproteins. Attributing to the formation of Fe-Zr bimetallic dual active centers, the as-prepared Fe 3 O 4 @ZNP@UiO-66 exhibits enhanced peroxidase-mimicking catalytic activity, which promotes the electron transfer from Zr center to Fe(II)/Fe(III). The catalytic activity of Fe 3 O 4 @ZNP@UiO-66 can be selectively inhibited by phosphoproteins due to the strong interaction between phosphate groups and Zr centers, as well as the ultra-robust antifouling capability of zwitterionic dopamine nanoparticle (ZNP). Considering the diverse binding affinities between various proteins with the nanozyme, the catalytic activity of Fe 3 O 4 @ZNP@UiO-66 can be changed to various degree, leading to the different absorption responses at 420 nm in the hydrogen peroxide (H 2 O 2 ) - 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) system. By simply extracting different absorbance intensities at various time points, a sensor array based on reaction kinetics for the discrimination of phosphoproteins from other proteins is constructed through linear discriminant analysis (LDA). Besides, the quantitative determination of phosphoproteins and identification of protein mixtures have been realized. Further, based on the differential level of phosphoproteins in cells, the differentiation of cancer cells from normal cells can also be implemented by utilizing the proposed sensor array, showing great potential in disease diagnosis.
      Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2024 Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: Dual active centers; Peroxidase‐mimicking nanozyme; Phosphoproteins discrimination; Sensor array; Zwitterionic polymers
    • Publication Date:
      Date Created: 20240514 Date Completed: 20240519 Latest Revision: 20240519
    • Publication Date:
      20240520
    • Accession Number:
      10.1016/j.bios.2024.116370
    • Accession Number:
      38744115