Mesencephalic astrocyte-derived neurotrophic factor inhibits cervical cancer progression via regulating macrophage phenotype.

Publisher: Reidel Country of Publication: Netherlands NLM ID: 0403234 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-4978 (Electronic) Linking ISSN: 03014851 NLM ISO Abbreviation: Mol Biol Rep Subsets: MEDLINE

Original Publication: Dordrecht, Boston, Reidel.

Macrophages*/metabolism ; Nerve Growth Factors*/metabolism ; Nerve Growth Factors*/genetics ; Uterine Cervical Neoplasms*/genetics ; Uterine Cervical Neoplasms*/pathology ; Uterine Cervical Neoplasms*/metabolism; Animals ; Female ; Humans ; Mice ; Cell Line, Tumor ; Cell Movement/genetics ; Cell Proliferation ; Disease Progression ; Epithelial-Mesenchymal Transition/genetics ; Gene Expression Regulation, Neoplastic ; HeLa Cells ; Mice, Nude ; NF-kappa B/metabolism ; Phenotype ; Signal Transduction

Background: Cervical cancer is a common gynecologic malignant tumor, but the critical factors affecting cervical cancer progression are still not well demonstrated. Mesencephalic astrocyte-derived neurotrophic factor (MANF) has been widely recognized as an anti-inflammatory factor to regulate macrophage polarization. In this study, the effect and mechanism of MANF on cervical cancer were preliminarily explored.
Methods and Results: Kaplan-Meier curve was used to show the overall survival time of the involved cervical cancer patients with high and low MANF expression in cervical cancer tissues. MANF was highly expressed in peritumoral tissues of cervical carcinoma by using immunohistochemistry and western blot. MANF mRNA level was detected by using qRT-PCR. Dual-labeled immunofluorescence showed MANF was mainly expressed in macrophages of cervical peritumoral tissues. Moreover, MANF-silenced macrophages promoted HeLa and SiHa cells survival, migration, invasion and EMT via NF-κB signaling activation. The results of tumor formation in nude mice indicated MANF-silenced macrophages promoted cervical tumor formation in vivo.
Conclusion: Our study reveals an inhibitory role of MANF in cervical cancer progression, indicating MANF as a new and valuable therapeutic target for cervical cancer treatment.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)

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Keywords: Cervical cancer; Macrophage differentiation; Mesencephalic astrocyte-derived neurotrophic factor; Nuclear factor-kappa B

0 (MANF protein, human)
0 (Nerve Growth Factors)
0 (NF-kappa B)

Date Created: 20240512 Date Completed: 20240512 Latest Revision: 20240708

20240709

10.1007/s11033-024-09602-6

38735002