Preparation, characterization and evaluation of HPβCD-PTX/PHB nanoparticles for pH-responsive, cytotoxic and apoptotic properties.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE
    • Publication Information:
      Publication: Amsterdam : Elsevier
      Original Publication: Guildford, Eng., IPC Science and Technology Press.
    • Subject Terms:
      Nanoparticles*/chemistry ; Paclitaxel*/pharmacology ; Paclitaxel*/chemistry ; Apoptosis*/drug effects ; 2-Hydroxypropyl-beta-cyclodextrin*/chemistry ; Prohibitins* ; Drug Carriers*/chemistry ; Polyesters*/chemistry; Humans ; Hydrogen-Ion Concentration ; MCF-7 Cells ; Hydroxybutyrates/chemistry ; Hydroxybutyrates/pharmacology ; Cell Line, Tumor ; Drug Liberation ; Solubility ; Cell Survival/drug effects ; Polyhydroxybutyrates
    • Abstract:
      Paclitaxel (PTX) is a potent anticancer drug. However, PTX exhibits extremely poor solubility in aqueous solution along with severe side effects. Therefore, in this study, an inclusion complex was prepared between PTX and hydroxypropyl-β-cyclodextrin (HPβCD) by solvent evaporation to enhance the drug's solubility. The HPβCD-PTX inclusion complex was then encapsulated in poly-3-hydroxybutyrate (PHB) to fabricate drug-loaded nanoparticles (HPβCD-PTX/PHB NPs) by nanoprecipitation. The HPβCD-PTX/PHB NPs depicted a higher release of PTX at pH 5.5 thus demonstrating a pH-dependent release profile. The cytotoxic properties of HPβCD-PTX/PHB NPs were tested against MCF-7, MDA-MB-231 and SW-620 cell lines. The cytotoxic potential of HPβCD-PTX/PHB NPs was 2.59-fold improved in MCF-7 cells in comparison to free PTX. Additionally, the HPβCD-PTX/PHB NPs improved the antimitotic (1.68-fold) and apoptotic (8.45-fold) effects of PTX in MCF-7 cells in comparison to PTX alone. In summary, these pH-responsive nanoparticles could be prospective carriers for enhancing the cytotoxic properties of PTX for the treatment of breast cancer.
      Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
      (Copyright © 2024 Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: HPβCD-drug inclusion complex; Nano-system; PHB; Paclitaxel
    • Accession Number:
      P88XT4IS4D (Paclitaxel)
      0 (PHB protein, human)
      1I96OHX6EK (2-Hydroxypropyl-beta-cyclodextrin)
      0 (Prohibitins)
      0 (Drug Carriers)
      0 (Polyesters)
      0 (Hydroxybutyrates)
      26063-00-3 (poly-beta-hydroxybutyrate)
      0 (Polyhydroxybutyrates)
    • Publication Date:
      Date Created: 20240511 Date Completed: 20240604 Latest Revision: 20241008
    • Publication Date:
      20241008
    • Accession Number:
      10.1016/j.ijbiomac.2024.132268
    • Accession Number:
      38734336