Taxonomic characterization of Sphaerotilus microaerophilus sp. nov., a sheath-forming microaerophilic bacterium of activated sludge origin.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 0410427 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-072X (Electronic) Linking ISSN: 03028933 NLM ISO Abbreviation: Arch Microbiol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Berlin, New York, Springer-Verlag.
    • Subject Terms:
      Sewage*/microbiology ; RNA, Ribosomal, 16S*/genetics ; Phylogeny* ; Base Composition* ; Fatty Acids*/analysis ; DNA, Bacterial*/genetics; Bacterial Typing Techniques ; Sequence Analysis, DNA ; Japan ; Genome, Bacterial
    • Abstract:
      A microaerophilic Gram-stain-negative bacilliform bacterial strain, FB-5  T , was isolated from activated sludge in Yokohama, Japan, that exhibited filamentous growth and formed a microtube (sheath). Cells were motile using a single polar flagellum. The optimum growth temperature and pH were 30 °C and 7.5, respectively. Strain FB-5  T was catalase-negative. Peptides and amino acids were utilized as energy and carbon sources. Sugars and organic acids were not utilized. Vitamin B 12 enhanced the growth of strain FB-5  T . Sulfur-dependent lithotrophic growth was possible. Major respiratory quinone was UQ-8. Major fatty acids were C 16:1 ω7 and C 16:0 . The genomic DNA G + C content was 69.16%. Phylogenetic analysis of the 16S rRNA gene suggested that strain FB-5  T belongs to the genus Sphaerotilus. The close relatives were S. natans subsup. sulfidivorans and S. natans subsup. natans with 98.0% and 97.8% similarity based on the 16S rRNA gene analysis, respectively. The genome size (6.06 Mbp) was larger than that (4.39-5.07 Mbp) of the Sphaerotilus strains. The AAI values against the related strains ranged from 71.0 to 72.5%. The range of ANI values was 81.7 - 82.5%. In addition to these distinguishable features of the genome, the core genome and dDDH analyses suggested that this strain is a novel member of the genus Sphaerotilus. Based on its physiological properties and genomic features, strain FB-5  T is considered as a novel species of the genus Sphaerotilus, for which the name S. microaerophilus sp. nov. is proposed. The type strain is FB-5  T (= JCM 35424  T  = KACC 23146  T ).
      (© 2024. The Author(s).)
    • References:
      Nat Commun. 2018 Nov 30;9(1):5114. (PMID: 30504855)
      Syst Appl Microbiol. 2013 Feb;36(1):69-73. (PMID: 23410935)
      Front Microbiol. 2021 Jun 24;12:691563. (PMID: 34248917)
      J Bacteriol. 1964 Feb;87:286-92. (PMID: 14151046)
      FEMS Microbiol Ecol. 2014 Oct;90(1):68-77. (PMID: 24965827)
      BMC Bioinformatics. 2013 Feb 21;14:60. (PMID: 23432962)
      Curr Microbiol. 2011 Aug;63(2):173-80. (PMID: 21643851)
      Water Res. 2003 May;37(9):1991-2000. (PMID: 12691883)
      Brief Bioinform. 2019 Jul 19;20(4):1160-1166. (PMID: 28968734)
      J Microbiol. 2021 May;59(5):476-480. (PMID: 33907973)
      Appl Environ Microbiol. 1992 Dec;58(12):4001-10. (PMID: 16348826)
      Microbiol Rev. 1978 Jun;42(2):329-56. (PMID: 353479)
      Bioinformatics. 2015 Oct 15;31(20):3350-2. (PMID: 26099265)
      FEMS Microbiol Ecol. 2014 Nov;90(2):454-66. (PMID: 25098830)
      Int J Biol Macromol. 2021 Jul 31;183:992-1001. (PMID: 33964269)
      Syst Appl Microbiol. 2022 Nov;45(6):126352. (PMID: 36063784)
      Arch Microbiol. 2021 May;203(4):1595-1599. (PMID: 33399897)
      Appl Environ Microbiol. 2002 Jan;68(1):365-71. (PMID: 11772646)
      Astrobiology. 2012 Apr;12(4):327-46. (PMID: 22519973)
      PLoS One. 2020 Jan 22;15(1):e0227567. (PMID: 31968006)
      J Bacteriol. 1991 Jan;173(2):697-703. (PMID: 1987160)
      Lett Appl Microbiol. 2009 Sep;49(3):396-402. (PMID: 19627475)
      Int J Biol Macromol. 2011 Mar 1;48(2):256-62. (PMID: 21095202)
      Mol Plant. 2020 Aug 3;13(8):1194-1202. (PMID: 32585190)
      Biosci Microbiota Food Health. 2016;35(4):173-184. (PMID: 27867804)
      Int J Syst Evol Microbiol. 2023 Dec;73(11):. (PMID: 38038645)
      Appl Environ Microbiol. 2023 Apr 26;89(4):e0191922. (PMID: 36951572)
      Int J Biol Macromol. 2005 Oct 30;37(1-2):92-8. (PMID: 16214212)
      Nat Methods. 2023 Aug;20(8):1203-1212. (PMID: 37500759)
      Nucleic Acids Res. 2022 Jan 7;50(D1):D801-D807. (PMID: 34634793)
      Mol Biol Evol. 2020 May 1;37(5):1530-1534. (PMID: 32011700)
      J Microbiol. 2018 Apr;56(4):280-285. (PMID: 29492869)
      Int J Syst Evol Microbiol. 2002 May;52(Pt 3):895-900. (PMID: 12054255)
      Int J Syst Evol Microbiol. 2007 Jan;57(Pt 1):81-91. (PMID: 17220447)
      Genome Res. 2015 Jul;25(7):1043-55. (PMID: 25977477)
      Int J Syst Evol Microbiol. 2011 Apr;61(Pt 4):916-925. (PMID: 20495027)
      Mol Biol Evol. 2021 Jun 25;38(7):3022-3027. (PMID: 33892491)
      PLoS One. 2010 Mar 10;5(3):e9490. (PMID: 20224823)
      Biosci Biotechnol Biochem. 2023 Feb 24;87(3):256-266. (PMID: 36535637)
      Int J Syst Bacteriol. 1996 Jan;46(1):173-82. (PMID: 8573492)
      Nat Methods. 2017 Jun;14(6):587-589. (PMID: 28481363)
    • Grant Information:
      23K05052 Japan Society for the Promotion of Science
    • Contributed Indexing:
      Keywords: Sphaerotilus microaerophilus sp. nov.; Activated sludge; Microaerophile; Sheath
    • Accession Number:
      0 (Sewage)
      0 (RNA, Ribosomal, 16S)
      0 (Fatty Acids)
      0 (DNA, Bacterial)
    • Publication Date:
      Date Created: 20240510 Date Completed: 20240510 Latest Revision: 20240619
    • Publication Date:
      20240619
    • Accession Number:
      PMC11087309
    • Accession Number:
      10.1007/s00203-024-03991-9
    • Accession Number:
      38727820