LncRNA LINC00466 Promotes the Progression of Breast Cancer via miR-4731-5p/EPHA2 Pathway.

Publisher: Bentham Science Publishers Country of Publication: Netherlands NLM ID: 100960530 Publication Model: Print Cited Medium: Internet ISSN: 1873-4316 (Electronic) Linking ISSN: 13892010 NLM ISO Abbreviation: Curr Pharm Biotechnol Subsets: MEDLINE

Original Publication: Hilversum, Netherlands ; Boca Raton, FL : Bentham Science Publishers, c2000-

RNA, Long Noncoding*/genetics ; RNA, Long Noncoding*/metabolism ; Breast Neoplasms*/genetics ; Breast Neoplasms*/pathology ; Breast Neoplasms*/metabolism ; MicroRNAs*/genetics ; MicroRNAs*/metabolism ; Receptor, EphA2*/genetics ; Receptor, EphA2*/metabolism ; Cell Proliferation*/genetics ; Mice, Nude*; Humans ; Female ; Animals ; Mice ; Cell Line, Tumor ; Mice, Inbred BALB C ; Gene Expression Regulation, Neoplastic ; Disease Progression

Background: Breast Cancer (BC) is a female malignancy with a high mortality rate. Novel diagnostic and prognostic biomarkers are valuable for reducing BC mortality. Our study is designed to undrape the precise role of the LINC00466/miR-4731-5p/EPHA2 axis in BC.
Methods: The Cancer Genome Atlas (TCGA) sequencing dataset was utilized to compare the levels of LINC00466. The levels of LINC00466, miR-4731-5p, and EPHA2 were tested by qRTPCR. Cell proliferation and cycle were detected by CCK-8 assay and flow cytometer. In vivo role of LINC00466 was tested by Xenograft nude models. Binding sites were predicted by TargetScan and Starbase. The binding relationship was employed by Dual-luciferase reporter gene assay and RNA pull-down assay.
Results: LINC00466 was increased in human breast cancer tissues. LINC00466 was negatively associated with miR-4731-5p and positively correlated with EPHA2 in human breast cancer tissues. Down-regulation of LINC00466 suppressed the proliferation and arrested the cell cycle of breast cancer cells, and inhibited tumor growth in vivo .
Conclusion: LINC00466 promoted BC development via mediating the miR-4731-5p/EPHA2 axis, which has the potential value as a promising therapeutic target in BC.
(Copyright© Bentham Science Publishers; For any queries, please email at [email protected].)

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2008085QH410 Anhui Provincial Natural Science Foundation; KJ2021A0725 Key Project of Natural Science Research in Higher Education Institutions of Anhui Province; KJ2020ZD49 Major University Science Research Project of Anhui Province; S202010367101 Anhui Provincial College Students Innovation and Entrepreneurship Project; bydc2022030 College Students Innovation and Entrepreneurship Project of Bengbu Medical College; BYKY1804ZD, 2020bypd009 Natural Science Research Program of Bengbu Medical College

Keywords: Breast cancer; EPHA2; LINC00466; Xenograft nude models; biomarkers.; miR-4731-5p

0 (RNA, Long Noncoding)
0 (MicroRNAs)
EC 2.7.10.1 (Receptor, EphA2)
0 (EPHA2 protein, human)

Date Created: 20240510 Date Completed: 20241129 Latest Revision: 20241129

20241202

10.2174/0113892010290582240419051056

38726776