Collagen degradation assessment with an in vitro rotator cuff tendinopathy model using multiparametric ultrashort-TE magnetization transfer (UTE-MT) imaging.

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  • Additional Information
    • Source:
      Publisher: Wiley Country of Publication: United States NLM ID: 8505245 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-2594 (Electronic) Linking ISSN: 07403194 NLM ISO Abbreviation: Magn Reson Med Subsets: MEDLINE
    • Publication Information:
      Publication: 1999- : New York, NY : Wiley
      Original Publication: San Diego : Academic Press,
    • Subject Terms:
      Tendinopathy*/diagnostic imaging ; Tendinopathy*/metabolism ; Collagen*/metabolism ; Rotator Cuff*/diagnostic imaging ; Rotator Cuff*/metabolism ; Magnetic Resonance Imaging*/methods; Humans ; Male ; Female ; Middle Aged ; Aged ; Collagenases/metabolism ; Tendons/diagnostic imaging ; Tendons/metabolism ; Image Processing, Computer-Assisted/methods
    • Abstract:
      Purpose: This study aims to assess ultrashort-TE magnetization transfer (UTE-MT) imaging of collagen degradation using an in vitro model of rotator cuff tendinopathy.
      Methods: Thirty-six supraspinatus tendon specimens were divided into three groups and treated with 600 U collagenase (Group 1), 150 U collagenase (Group 2), and phosphate buffer saline (Group 3). UTE-MT imaging was performed to assess changes in macromolecular fraction (MMF), macromolecule transverse relaxation time (T 2m ), water longitudinal relaxation rate constant (R 1m ), the magnetization exchange rate from the macromolecular to water pool (Rm 0 w ) and from water to the macromolecular pool (Rm 0 m ), and magnetization transfer ratio (MTR) at baseline and following digestion and their differences between groups. Biochemical and histological studies were conducted to determine the extent of collagen degradation. Correlation analyses were performed with MMF, T 2m , R 1m , Rm 0 w , Rm 0 m , and MTR, respectively. Univariate and multivariate linear regression analyses were performed to evaluate combinations of UTE-MT parameters to predict collagen degradation.
      Results: MMF, T 2m , R 1m , Rm 0 m , and MTR decreased after digestion. MMF (r = -0.842, p < 0.001), MTR (r = -0.78, p < 0.001), and Rm 0 m (r = -0.662, p < 0.001) were strongly negatively correlated with collagen degradation. The linear regression model of differences in MMF and Rm 0 m before and after digestion explained 68.9% of collagen degradation variation in the tendon. The model of postdigestion in MMF and T 2m and the model of MTR explained 54.2% and 52.3% of collagen degradation variation, respectively.
      Conclusion: This study highlighted the potential of UTE-MT parameters for evaluation of supraspinatus tendinopathy.
      (© 2024 International Society for Magnetic Resonance in Medicine.)
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    • Grant Information:
      BJ-2023-108 National High Level Hospital Clinical Research Funding; 81801673 National Natural Science Foundation of China
    • Contributed Indexing:
      Keywords: UTE; collagen degradation; magnetization transfer; rotator cuff tendinopathy
    • Accession Number:
      9007-34-5 (Collagen)
      EC 3.4.24.- (Collagenases)
    • Publication Date:
      Date Created: 20240510 Date Completed: 20240722 Latest Revision: 20240822
    • Publication Date:
      20240822
    • Accession Number:
      10.1002/mrm.30144
    • Accession Number:
      38725197